HIV-1感染细胞中小非编码RNA的焦磷酸测序：病毒-细胞双链RNA杂交体加工的证据
Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid.
作者信息
Yeung Man Lung, Bennasser Yamina, Watashi Koichi, Le Shu-Yun, Houzet Laurent, Jeang Kuan-Teh
机构信息
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0460, USA.
出版信息
Nucleic Acids Res. 2009 Oct;37(19):6575-86. doi: 10.1093/nar/gkp707. Epub 2009 Sep 3.
Small non-coding RNAs of 18-25 nt in length can regulate gene expression through the RNA interference (RNAi) pathway. To characterize small RNAs in HIV-1-infected cells, we performed linker-ligated cloning followed by high-throughput pyrosequencing. Here, we report the composition of small RNAs in HIV-1 productively infected MT4 T-cells. We identified several HIV-1 small RNA clones and a highly abundant small 18-nt RNA that is antisense to the HIV-1 primer-binding site (PBS). This 18-nt RNA apparently originated from the dsRNA hybrid formed by the HIV-1 PBS and the 3' end of the human cellular tRNAlys3. It was found to associate with the Ago2 protein, suggesting its possible function in the cellular RNAi machinery for targeting HIV-1.
长度为18 - 25个核苷酸的小非编码RNA可通过RNA干扰（RNAi）途径调节基因表达。为了鉴定HIV-1感染细胞中的小RNA，我们进行了连接子连接克隆，随后进行高通量焦磷酸测序。在此，我们报告了HIV-1高效感染的MT4 T细胞中小RNA的组成。我们鉴定出了几个HIV-1小RNA克隆以及一种高度丰富的18个核苷酸的小RNA，它与HIV-1引物结合位点（PBS）呈反义关系。这种18个核苷酸的RNA显然起源于由HIV-1 PBS和人类细胞tRNAlys3的3'末端形成的双链RNA杂交体。发现它与Ago2蛋白相关联，表明其在靶向HIV-1的细胞RNAi机制中可能具有功能。
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