Ahmed Z, Lewis C A, Faber D S
Department of Physiology, State University of New York, Buffalo 14214.
Brain Res. 1990 May 14;516(1):165-9. doi: 10.1016/0006-8993(90)90914-w.
We report here that, in the absence of external Ca2+, glutamate produces a transient increase in intracellular Ca2+ [( Ca2+]i) in cultured spinal cord astrocytes, by mobilizing internal Ca2+ stores. [Ca2+]i was measured with Fura-2 using the ratiometric method. Astrocytes were identified by immunostaining for glial fibrillary acidic protein. Glutamate and quisqualate consistently caused a transient increase in [Ca2+]i, with both the response amplitude and latency being graded functions of agonist concentration. Some cells produced multiple transients. N-Methyl-D-aspartate, kainate and aspartate were ineffective. We conclude that the quisqualate subtype of glutamate receptors mediates release of Ca2+ from internal stores in mammalian astrocytes.
我们在此报告，在没有细胞外钙离子（Ca2+）的情况下，谷氨酸通过动员细胞内钙库，使培养的脊髓星形胶质细胞内的钙离子浓度（[Ca2+]i）产生短暂升高。使用比率测量法用Fura-2测量[Ca2+]i。通过对胶质纤维酸性蛋白进行免疫染色来鉴定星形胶质细胞。谷氨酸和喹氨酸始终会引起[Ca2+]i的短暂升高，反应幅度和潜伏期均为激动剂浓度的分级函数。一些细胞产生多个瞬变。N-甲基-D-天冬氨酸、海人酸和天冬氨酸无效。我们得出结论，谷氨酸受体的喹氨酸亚型介导哺乳动物星形胶质细胞内钙库中Ca2+的释放。
