Food and Drug Laboratory Research Center (FDLRC), Ministry of Health (MOH), Tehran, Iran.
Toxicol Mech Methods. 2009 Mar;19(3):246-50. doi: 10.1080/15376510802455347.
Zearalenone (Zen) is a mycotoxin with estrogenic effect which contaminates cereals. In cell culture, Zen and its metabolite, alpha-Zearalenol (alpha-Zel), stimulate breast cancer cells growth. Today hormone-dependent cancers are important because of high incidence and death rate. Previous studies showed that Zen and alpha-Zel have an effect on hormone-dependent cancers. This study explains the effects of the mentioned compounds in comparison with Raloxifene as an anti-estrogen. Cell culture technique was used with MDA-MB-231 and T47D cells for evaluation of compounds. MDA-MB-231 cells were used as negative control and also for proving that treatment compounds merely affect, due to their proliferation activity in the applied doses. According to the Resazurine-based method, for toxicity assay, none of the test compounds have an effect on MDA-MB-231 cells but do effect the growth of T47D cells. Zen and alpha-Zel at low concentrations (10-8-10-9 M) stimulated T47D cell growth and Raloxifene strongly inhibited cell growth induced by Zen and alpha-Zel. There is a noticeable result in controlling diet of hormonal carcinogenic compounds and applying novel anti-estrogens for prevention and treatment of hormone-dependent cancers.
玉米赤霉烯酮（Zen）是一种具有雌激素作用的真菌毒素，会污染谷物。在细胞培养中，Zen 及其代谢物α-Zearalenol（α-Zel）会刺激乳腺癌细胞生长。如今，由于发病率和死亡率高，激素依赖性癌症变得尤为重要。先前的研究表明，Zen 和α-Zel 对激素依赖性癌症有影响。本研究将这些化合物与雷洛昔芬（一种抗雌激素）进行比较，以解释其作用。使用 MDA-MB-231 和 T47D 细胞的细胞培养技术来评估这些化合物。MDA-MB-231 细胞用作阴性对照，也证明了由于其在应用剂量下的增殖活性，处理化合物仅会产生影响。根据 Resazurine 法进行毒性测定，在 MDA-MB-231 细胞中，没有一种测试化合物具有作用，但会影响 T47D 细胞的生长。Zen 和α-Zel 在低浓度（10-8-10-9 M）下刺激 T47D 细胞生长，而雷洛昔芬则强烈抑制 Zen 和α-Zel 诱导的细胞生长。控制饮食中的激素致癌化合物并应用新型抗雌激素来预防和治疗激素依赖性癌症，是一项值得注意的成果。
