State Museum of Natural History, Rosenstein 1, Stuttgart, Germany.
Mycorrhiza. 2010 Mar;20(3):147-59. doi: 10.1007/s00572-009-0275-9.
Aneura pinguis (Aneuraceae) is a cosmopolitan thalloid liverwort that shows a specific mycorrhiza-like interaction with basidiomycetes. To date, tropical specimens have not been studied in great depth. Samples of A. pinguis were collected from 48 individuals in one plot in South Ecuador and 54 individuals in five European countries. Light and transmission electron microscopy and molecular analyses based on nuclear rDNA coding for the ribosomal large subunit (nucLSU) and from the 5.8s-ITS2 regions were carried out to identify the associated mycobionts and to study their phylogenetic relationships. Microscopic and ultrastructural investigations of the fungal colonisation showed a high congruence between the European and the Ecuadorian sites and confirmed previous results. Tulasnellales are the only mycobionts that could be detected from ultrastructural characters with certainty. Molecular phylogenetic analysis indicated the presence of tulasnelloid fungi from at least 13 distinct clades. The composition of the communities of tulasnelloid fungi in A. pinguis differs between Ecuador and Europe. The diversity of tulasnelloid fungal partners was much higher at the Ecuadorian site.
平腹足苔（Aneuraceae）是一种世界性的叶状苔类植物，与担子菌门真菌具有特定的菌根样相互作用。迄今为止，对热带标本的研究还不够深入。从厄瓜多尔南部的一个样地的 48 个个体和五个欧洲国家的 54 个个体中采集了平腹足苔的样本。利用基于核糖体大亚基（nucLSU）编码核 rDNA 和 5.8s-ITS2 区的光镜和透射电子显微镜以及分子分析来鉴定相关的菌根真菌，并研究它们的系统发育关系。对真菌定殖的微观和超微结构研究表明，欧洲和厄瓜多尔的地点之间具有高度一致性，并证实了先前的结果。只有从超微结构特征才能确定地检测到 Tulasnellales 是菌根真菌。分子系统发育分析表明，至少有 13 个不同的分支存在着 Tulasnelloid 真菌。平腹足苔中 Tulasnelloid 真菌群落的组成在厄瓜多尔和欧洲有所不同。厄瓜多尔地点的 Tulasnelloid 真菌伙伴的多样性要高得多。
