CRUK Beatson Institute, Bearsden, Glasgow, UK.
Trends Cell Biol. 2009 Oct;19(10):523-30. doi: 10.1016/j.tcb.2009.07.004. Epub 2009 Sep 3.
Understanding cell migration is centrally important to modern cell biology. However, despite years of study, progress has been hindered by experimental limitations and the complexity of the process. This has led to the popularity of Dictyostelium discoideum, with its experimentally-friendly lifestyle and small, haploid genome, as a tool to dissect the pathways involved in migration. This humble amoeba is now established at the centre of dramatic changes in our understanding of cell movement. In this review we describe the recent reinterpretation of the role of phosphatidylinositol trisphosphate (PIP(3)) and other intracellular messengers that connect signalling and migration, and the transition to models of chemotaxis driven by multiple, intertwined signalling pathways. In shallow gradients, pseudopods are generated with random directions, and we discuss how chemotaxis can operate by biasing this process. Overall we describe how Dictyostelium has the potential to unlock many fundamental questions in the cell motility field.
理解细胞迁移对于现代细胞生物学至关重要。然而，尽管经过多年的研究，由于实验限制和过程的复杂性，进展一直受到阻碍。这导致了 Dictyostelium discoideum 的流行，它具有实验友好的生活方式和小的、单倍体基因组，是剖析参与迁移的途径的工具。这种不起眼的变形虫现在成为了我们对细胞运动理解的戏剧性变化的中心。在这篇综述中，我们描述了最近对磷脂酰肌醇三磷酸（PIP(3)）和其他连接信号转导和迁移的细胞内信使的作用的重新解释，以及向由多个相互交织的信号通路驱动的趋化性模型的转变。在浅梯度中，伪足随机生成，我们讨论了趋化性如何通过偏向这个过程来起作用。总的来说，我们描述了 Dictyostelium 如何有可能解开细胞运动领域的许多基本问题。
