Markovic Danijela, Grammatopoulos Dimitris K
GPCR Pathophysiology Group, Division of Endocrinology and Metabolism, Clinical Sciences Research Institute, Warwick Medical School, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
Trends Biochem Sci. 2009 Sep;34(9):443-52. doi: 10.1016/j.tibs.2009.06.002. Epub 2009 Sep 3.
The family of G-protein coupled receptors (GPCRs) is one of the largest protein families in the mammalian genome with a fundamental role in cell biology. GPCR activity is finely tuned by various transcriptional, post-transcriptional and post-translational mechanisms. Alternative pre-mRNA splicing is now emerging as a crucial process regulating GPCR biological function. Intriguingly, this mechanism appears to extensively target the Secretin family of GPCRs, especially the exon that encodes a 14 amino acid sequence that forms the distal part of 7th transmembrane helix, and exhibits an unusually high level of sequence conservation among most Secretin GPCRs. Do the "TMD7-short" receptor variants have a role as novel regulators of GPCR signallng and, if so, what are the implications for hormonal actions and physiology?
G蛋白偶联受体（GPCRs）家族是哺乳动物基因组中最大的蛋白质家族之一，在细胞生物学中具有重要作用。GPCR的活性通过各种转录、转录后和翻译后机制进行精细调节。可变前体mRNA剪接正逐渐成为调节GPCR生物学功能的关键过程。有趣的是，这种机制似乎广泛作用于GPCR的促胰液素家族，特别是编码形成第7个跨膜螺旋远端部分的14个氨基酸序列的外显子，并且在大多数促胰液素GPCR中表现出异常高的序列保守性。“TMD7-短”受体变体是否作为GPCR信号传导的新型调节因子发挥作用，如果是，对激素作用和生理学有何影响？
