Zhang Xueyou, Xiao Qihai, Tian Kai, Wang Yan, Zhao Xiaoling, Yin Huadong, Li Diyan, Zhu Qing
Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
Biomed Res Int. 2017;2017:1074054. doi: 10.1155/2017/1074054. Epub 2017 Jan 22.
is a G protein-coupled receptor that plays critical roles in eukaryotic cells: typically, response to glucose stimulation, lipid accumulation, and transmitting nutrition signals to cAMP pathway. However, the alternative splicing of the gene and its expression pattern in chicken tissues and ovarian follicles were unknown. In our current study, we used RACE-PCR to identify three variants, including the full-length variant () and two alternatively spliced variants (, ). Quantitative real-time PCR examined the expression pattern of mRNA in chicken tissues and ovarian follicles. The result reveals that the coding sequence of the three variants cDNA is 1053, 1053, and 627 bp in length, encoding 350, 350, and 208 amino acids, respectively. The three variants of show similar tissue distributions; expression was abundant in the abdominal fat, lung, and heart. With the follicular development, the expression of gene gradually increased, and and spliced variants expression in F2 were significantly higher than in F5, F4, and prehierarchical follicles ( < 0.05). Taken together, we found three novel variants of , and the results of expression profiling in adipose tissues and ovarian follicles suggest that may play a significant role in the lipid accumulation and progression of follicular development.
是一种G蛋白偶联受体，在真核细胞中发挥关键作用：通常对葡萄糖刺激作出反应、参与脂质积累，并将营养信号传递至cAMP途径。然而，该基因在鸡组织和卵泡中的可变剪接及其表达模式尚不清楚。在我们当前的研究中，我们使用RACE-PCR鉴定了三个变体，包括全长变体（）和两个可变剪接变体（，）。实时定量PCR检测了该mRNA在鸡组织和卵泡中的表达模式。结果显示，三个变体cDNA的编码序列长度分别为1053、1053和627bp，分别编码350、350和208个氨基酸。该基因的三个变体显示出相似的组织分布；在腹部脂肪、肺和心脏中表达丰富。随着卵泡发育，该基因的表达逐渐增加，并且F2中、剪接变体的表达显著高于F5、F4和等级前卵泡（<0.05）。综上所述，我们发现了该基因的三个新变体，并且在脂肪组织和卵泡中的表达谱结果表明该基因可能在脂质积累和卵泡发育进程中发挥重要作用。
