Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Laboratorio de Biología de Virus, Caracas 1020A, Venezuela.
Diagn Microbiol Infect Dis. 2009 Nov;65(3):247-53. doi: 10.1016/j.diagmicrobio.2009.07.022. Epub 2009 Sep 5.
The performances of 2 commercial enzyme-linked immunosorbent assay (ELISA) kits (PLATELIA Dengue NS1 AG and Dengue Early ELISA) and a rapid immunochromatography test (Dengue NS1 AG Strip) for detection of dengue NS1 protein were compared using a panel of 87 sera from viremic dengue patients, as well as 36 sera from patients with other acute febrile illnesses. PLATELIA was more sensitive and slightly less specific than Dengue Early ELISA (sensitivity, 71.3% versus 60.9%; specificity, 86.1% versus 94.3%, respectively). The strip test showed an overall sensitivity of 67.8% with a specificity of 94.4%. A lower sensitivity was observed with Dengue Early ELISA for dengue virus (DENV) type 4 (30%) and by the 3 tests for DENV type 2 (56.5%). The use of these kits allows for rapid and specific early diagnosis of dengue infection; however, their sensitivity for each serotype must be further evaluated to guarantee an accurate diagnosis, particularly in those regions where the 4 dengue serotypes are cocirculating.
比较了两种商业酶联免疫吸附测定（ELISA）试剂盒（PLATELIA Dengue NS1 AG 和 Dengue Early ELISA）和一种快速免疫层析检测（Dengue NS1 AG Strip）对登革热 NS1 蛋白的检测性能，使用了 87 份来自病毒血症登革热患者的血清样本和 36 份来自其他急性发热疾病患者的血清样本。PLATELIA 比 Dengue Early ELISA 更敏感，特异性略低（敏感性分别为 71.3%和 60.9%；特异性分别为 86.1%和 94.3%）。条带检测的总敏感性为 67.8%，特异性为 94.4%。对于登革热病毒（DENV）4 型，Dengue Early ELISA 的敏感性较低（30%），而 3 种检测方法对 DENV 2 型的敏感性也较低（56.5%）。这些试剂盒可用于快速特异性的早期登革热感染诊断；然而，为了保证准确诊断，还需要进一步评估它们对每种血清型的敏感性，特别是在存在 4 种登革热血清型共存的地区。
