一种商用登革热NS1抗原免疫层析快速检测法相对于登革热NS1抗原捕获酶联免疫吸附测定法的性能。
Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA.
作者信息
Zainah S, Wahab A H Abdul, Mariam M, Fauziah M K, Khairul A H, Roslina I, Sairulakhma A, Kadimon S S, Jais M S Mat, Chua K B
机构信息
Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
出版信息
J Virol Methods. 2009 Feb;155(2):157-60. doi: 10.1016/j.jviromet.2008.10.016. Epub 2008 Dec 2.
The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial dengue NS1 antigen-capture ELISA. The rapid immunochromatography test gave an overall sensitivity of 90.4% with a specificity of 99.5%. The sensitivity was highest for serum samples from which virus was isolated (96.3%) and lowest for those from which virus was not isolated and RT-PCR was negative (76.4%). The sensitivity was significantly higher for serum samples from patients with acute primary dengue (92.3%) than those from patients with acute secondary dengue (79.1%). The positive predictive value and negative predictive value of this commercial immunochromatography test were 99.6% and 87.9% respectively.
针对一种商业登革热NS1抗原捕获酶联免疫吸附测定（ELISA），对一种用于快速检测患者血清或血浆中登革热NS1抗原的商业免疫层析试验的性能进行了评估。这种快速免疫层析试验的总体灵敏度为90.4%，特异性为99.5%。对于分离出病毒的血清样本，灵敏度最高（96.3%），而对于未分离出病毒且逆转录聚合酶链反应（RT-PCR）为阴性的样本，灵敏度最低（76.4%）。急性原发性登革热患者的血清样本灵敏度（92.3%）显著高于急性继发性登革热患者的血清样本（79.1%）。这种商业免疫层析试验的阳性预测值和阴性预测值分别为99.6%和87.9%。
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