Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699, USA.
Biosens Bioelectron. 2009 Dec 15;25(4):695-701. doi: 10.1016/j.bios.2009.08.014. Epub 2009 Aug 14.
Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for "digital" (threshold) sensing/actuation. We outline the response functions desirable for such applications and report the first experimental realization of a sigmoid-shape response in one of the inputs. A kinetic model is developed and utilized to evaluate the extent to which the experimentally realized gate is close to optimal.
电极固定化葡萄糖-6-磷酸脱氢酶用于催化酶促反应，从而实现与门逻辑。在此，我们考虑了用于“数字”（阈值）传感/执行的生物计算应用中所利用的生物催化过程中的这种逻辑功能。我们概述了此类应用所需的响应函数，并报告了在其中一个输入中首次实现的 S 形响应的实验结果。我们开发了一个动力学模型，并利用该模型评估了实验实现的门接近最佳的程度。
