Department of Chemistry and Biochemistry, Center for Bioengineering, University of California, Santa Barbara, Santa Barbara, CA 93106, USA.
Angew Chem Int Ed Engl. 2012 Jul 2;51(27):6717-21. doi: 10.1002/anie.201202204. Epub 2012 Jun 5.
Here we demonstrate two convenient methods to extend and narrow the useful dynamic range of a model electrochemical DNA sensor. We did so by combining DNA probes of different target affinities but with similar specificity on the same electrode. We were able to achieve an extended dynamic response spanning 3 orders of magnitude in target concentration. Using a different strategy we have also narrowed the useful dynamic range of an E-DNA sensor to only an 8-fold range of target concentrations.
在这里，我们展示了两种方便的方法来扩展和缩小模型电化学生物传感器的有用动态范围。我们通过在同一电极上结合具有不同靶亲和力但具有相似特异性的 DNA 探针来实现这一点。我们能够实现扩展的动态响应，跨越 3 个数量级的目标浓度。使用不同的策略，我们还将 E-DNA 传感器的有用动态范围缩小到仅 8 倍的目标浓度范围。
