Lenz R, Alter A, Radnay J, Goldman J, Cooper O, Sredni D, Rozenszajn L A
Department of Life Sciences, Bar Ilan University, Ramat Gan, Israel.
Nat Immun Cell Growth Regul. 1990;9(3):228-36.
Blood mononuclear cells (MNC) develop into T cell colonies when the cells are sensitized with PHA and seeded in a two-layer soft agar system. Conditioned medium (CM) derived from MNC enhanced lymphocyte colony formation when it was added to the culture system. CFU-TL appear to be stimulated into colony formation by molecules secreted by lymphocyte subpopulations contained in the seeded cells. In this study, human peripheral blood MNC were fractionated by a battery of techniques into adherent, E+, CD4+, CD8+, B and null cells. CM was prepared from each of the subpopulations and its effects on T cell colony growth assayed. All the lymphocyte subpopulations were found to generate lymphocyte colony enhancement factor (LCEF). After several purification procedures, CM prepared from CD4 and CD8+, displayed LCEF activity corresponding to proteins of molecular weight 30-40 and 100-140 kD.
当用PHA致敏并接种于双层软琼脂系统中时，血液单核细胞（MNC）可发育成T细胞集落。当将来源于MNC的条件培养基（CM）添加到培养系统中时，可增强淋巴细胞集落形成。CFU-TL似乎受到接种细胞中所含淋巴细胞亚群分泌的分子刺激而形成集落。在本研究中，通过一系列技术将人外周血MNC分离为贴壁细胞、E+细胞、CD4+细胞、CD8+细胞、B细胞和裸细胞。从每个亚群制备CM，并检测其对T细胞集落生长的影响。发现所有淋巴细胞亚群均能产生淋巴细胞集落增强因子（LCEF）。经过几次纯化程序后，从CD4和CD8+制备的CM显示出与分子量为30-40kD和100-140kD的蛋白质相对应的LCEF活性。
