Zeevi A, Goldman I, Rozenszajn L A
Immunology. 1978 Mar;34(3):523-31.
The cloning potential of PHA-treated T cells is significantly enhanced when lymphocyte culture fluid (LCF) from mitogen-treated lymphocytes is added to the soft agar culture system. The mitogens seem to stimulate the release of a lymphocyte colony enhancing factor (LCEF) into the culture medium. A study of the physico-chemical properties of the LCEF revealed that it is a nondialyzable, heat-labile molecule which migrates in the haptoglobin (2--2) post-transferrin region in acrylamide electrophoresis. It is stable to RNase and DNase but labile to papain and trypsin. The LCEF was partially purified from the crude LCF using a sequence of techniques--ammonium sulphate precipitation, DEAE-cellulose and Bio-Gel P-150 chromatography and disc electrophoresis. The mol. wt of the purified LCEF, determined from gel filtration chromatography, was 90,000--110,000.
当将来自丝裂原处理淋巴细胞的淋巴细胞培养液（LCF）添加到软琼脂培养系统中时，PHA处理的T细胞的克隆潜力会显著增强。丝裂原似乎会刺激一种淋巴细胞集落增强因子（LCEF）释放到培养基中。对LCEF理化性质的研究表明，它是一种不可透析、热不稳定的分子，在丙烯酰胺电泳中迁移到触珠蛋白（2--2）转铁蛋白后区域。它对核糖核酸酶和脱氧核糖核酸酶稳定，但对木瓜蛋白酶和胰蛋白酶不稳定。使用一系列技术——硫酸铵沉淀、DEAE-纤维素和Bio-Gel P-150色谱法以及圆盘电泳，从粗制LCF中部分纯化了LCEF。通过凝胶过滤色谱法测定的纯化LCEF的分子量为90,000--110,000。
