Klein B, Caraux J, Thomas P, Goube De Laforest P
Immunology. 1982 Feb;45(2):265-71.
The mechanisms of action and the nature of the co-operating cells (CC) controlling human T-lymphocyte-colony formation were investigated. Media conditioned by PHA-stimulated blood mononuclear cells (MC) were tested for their capacity to induce T-colony formation in the effluent cell population, obtained after anti (Fab')2 cell affinity chromatography of MC. This cell population has been previously shown to still contain T-colony-forming cells (TCFC), but to be devoid of a co-operating cell population essential for T-cell-colony growth, thus requiring a feeder layer containing media conditioned by PHA-stimulated MC in order to generate T colonies. Further evidence is also presented that: phytohaemagglutinin (PHA) was necessary to two steps of T-colony formation: (i) for the production of colony promoting activity (CPA) by PHA-stimulated MC; (ii) for the induction of the TCFC to generate a colony, in the presence of CPA. There were high producers and low producers of CPA. The low CPA production observed with some donors could be explained by a suppressive effect mediated by phagocytic cells, presumably monocytes, whereas the cells retained on the anti-(Fab')2 immunoadsorbent (mainly B cells) were able to produce very high CPA levels.
对控制人T淋巴细胞集落形成的作用机制及协同细胞（CC）的性质进行了研究。检测了经PHA刺激的血液单核细胞（MC）条件培养基在MC经抗（Fab'）2细胞亲和层析后获得的流出细胞群体中诱导T集落形成的能力。先前已表明，该细胞群体仍含有T集落形成细胞（TCFC），但缺乏T细胞集落生长所必需的协同细胞群体，因此需要含有经PHA刺激的MC条件培养基的饲养层才能产生T集落。还提供了进一步的证据表明：植物血凝素（PHA）对T集落形成的两个步骤是必需的：（i）用于经PHA刺激的MC产生集落促进活性（CPA）；（ii）在存在CPA的情况下，用于诱导TCFC产生集落。存在CPA的高产者和低产者。一些供体观察到的低CPA产量可以用吞噬细胞（可能是单核细胞）介导的抑制作用来解释，而保留在抗（Fab'）2免疫吸附剂上的细胞（主要是B细胞）能够产生非常高的CPA水平。
