Wang Guirong, Qiu Yu T, Lu Tan, Kwon Hyung-Wook, Pitts R Jason, Van Loon Joop J A, Takken Willem, Zwiebel Laurence J
Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235, USA.
Eur J Neurosci. 2009 Sep;30(6):967-74. doi: 10.1111/j.1460-9568.2009.06901.x. Epub 2009 Sep 4.
Heat sensitivity is a sensory modality that plays a critical role in close-range host-seeking behaviors of adult female Anopheles gambiae, the principal Afrotropical vector for human malaria. An essential step in this activity is the ability to discriminate and respond to increases in environmental temperature gradients through the process of peripheral thermoreception. Here, we report on the characterization of the anopheline homolog of the transient receptor potential (TRP) A1/ANKTM1 channel that is consistent with its role as a heat-sensor in host-seeking adult female mosquitoes. We identify a set of distal antennal sensory structures that specifically respond to temperature gradients and express AgTRPA1. Functional characterization of AgTRPA1 in Xenopus oocytes supports its role in the molecular transduction of temperature gradients in An. gambiae, providing a basis for targeting mosquito heat responses as a means toward reducing malaria transmission.
热敏感性是一种感觉模态，在冈比亚按蚊成年雌蚊近距离寻找宿主的行为中起着关键作用，冈比亚按蚊是非洲热带地区人类疟疾的主要传播媒介。这一活动的一个重要步骤是能够通过外周温度感受过程来辨别环境温度梯度的增加并做出反应。在这里，我们报告了瞬时受体电位（TRP）A1/ANKTM1通道的按蚊同源物的特性，这与其作为寻找宿主的成年雌蚊热传感器的作用相一致。我们确定了一组远端触角感觉结构，它们对温度梯度有特异性反应并表达AgTRPA1。在非洲爪蟾卵母细胞中对AgTRPA1进行功能特性分析，支持了其在冈比亚按蚊温度梯度分子转导中的作用，为将蚊子的热反应作为减少疟疾传播的一种手段提供了靶点依据。
