RGS2介导的翻译调控。

Translational control by RGS2.

作者信息

Nguyen Chau H, Ming Hong, Zhao Peishen, Hugendubler Lynne, Gros Robert, Kimball Scot R, Chidiac Peter

机构信息

Department of Physiology and Pharmacology, The University of Western Ontario, London, Ontario N6A5C1, Canada.

出版信息

J Cell Biol. 2009 Sep 7;186(5):755-65. doi: 10.1083/jcb.200811058.

The regulator of G protein signaling (RGS) proteins are a family of guanosine triphosphatase (GTPase)-accelerating proteins. We have discovered a novel function for RGS2 in the control of protein synthesis. RGS2 was found to bind to eIF2Bepsilon (eukaryotic initiation factor 2B epsilon subunit) and inhibit the translation of messenger RNA (mRNA) into new protein. This effect was not observed for other RGS proteins tested. This novel function of RGS2 is distinct from its ability to regulate G protein-mediated signals and maps to a stretch of 37 amino acid residues within its conserved RGS domain. Moreover, RGS2 was capable of interfering with the eIF2-eIF2B GTPase cycle, which is a requisite step for the initiation of mRNA translation. Collectively, this study has identified a novel role for RGS2 in the control of protein synthesis that is independent of its established RGS domain function.

G蛋白信号调节（RGS）蛋白是一类鸟苷三磷酸酶（GTPase）加速蛋白。我们发现了RGS2在蛋白质合成控制中的新功能。研究发现RGS2与真核起始因子2Bε（eIF2Bε）结合，并抑制信使核糖核酸（mRNA）翻译成新蛋白质。在测试的其他RGS蛋白中未观察到这种效应。RGS2的这一新功能与其调节G蛋白介导信号的能力不同，定位于其保守RGS结构域内一段37个氨基酸残基的区域。此外，RGS2能够干扰eIF2-eIF2B GTPase循环，这是mRNA翻译起始的一个必要步骤。总的来说，这项研究确定了RGS2在蛋白质合成控制中的一个新作用，该作用独立于其已确定的RGS结构域功能。