Schotzinger R J, Landis S C
Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio.
Cell Tissue Res. 1990 May;260(3):575-87. doi: 10.1007/BF00297238.
Histochemical, immunocytochemical, and radioenzymatic techniques were used to examine the neurotransmitter-related properties of the innervation of thoracic hairy skin in rats during adulthood and postnatal development. In the adult, catecholamine-containing fibers were associated with blood vessels and piloerector muscles, and ran in nerve bundles throughout the dermis. The distribution of tyrosine hydroxylase (TH)-immunoreactive (IR) fibers was identical. Neuronal fibers displaying neuropeptide Y (NPY) immunoreactivity were seen in association with blood vessels. Double-labeling studies suggested that most, if not all, NPY-IR fibers were also TH-IR and likewise most, if not all, vessel-associated TH-IR fibers were also NPY-IR. Calcitonin gene-related peptide (CGRP)-IR fibers were observed near and penetrating into the epidermis, in close association with hair follicles and blood vessels, and in nerve bundles. A similar distribution of substance P (SP)-IR fibers was evident. In adult animals treated as neonates with the sympathetic neurotoxin 6-hydroxydopamine, a virtual absence of TH-IR and NPY-IR fibers was observed, whereas the distribution of CGRP-IR and SP-IR fibers appeared unaltered. During postnatal development, a generalized increase in the number, fluorescence intensity, and varicose morphology of neuronal fibers displaying catecholamine fluorescence, NPY-IR, CGRP-IR, and SP-IR was observed. By postnatal day 21, the distribution of the above fibers had reached essentially adult levels, although the density of epidermal-associated CGRP-IR and SP-IR fibers was significantly greater than in the adult. The following were not evident in thoracic hairy skin at any timepoint examined: choline acetyltransferase activity, acetylcholinesterase histochemical staining or immunoreactivity, fibers displaying immunoreactivity to vasoactive intestinal peptide, cholecystokinin, or leucine-enkephalin. The present study demonstrates that the thoracic hairy skin in developing and adult rats receives an abundant sympathetic catecholaminergic and sensory innervation, but not a cholinergic innervation.
采用组织化学、免疫细胞化学和放射酶法技术，研究成年期及出生后发育阶段大鼠胸部多毛皮肤神经支配的神经递质相关特性。在成年大鼠中，含儿茶酚胺的纤维与血管和竖毛肌相关，贯穿真皮并成束分布。酪氨酸羟化酶（TH）免疫反应性（IR）纤维的分布与之相同。可见显示神经肽Y（NPY）免疫反应性的神经元纤维与血管相关。双重标记研究表明，大多数（如果不是全部）NPY-IR纤维也是TH-IR纤维，同样，大多数（如果不是全部）与血管相关的TH-IR纤维也是NPY-IR纤维。降钙素基因相关肽（CGRP）-IR纤维在靠近并穿透表皮处被观察到，与毛囊和血管紧密相关，并存在于神经束中。P物质（SP）-IR纤维也有类似的分布。在用交感神经毒素6-羟基多巴胺处理过的新生期成年动物中，几乎观察不到TH-IR和NPY-IR纤维，而CGRP-IR和SP-IR纤维的分布似乎未改变。在出生后发育过程中，观察到显示儿茶酚胺荧光、NPY-IR、CGRP-IR和SP-IR的神经元纤维数量、荧光强度和曲张形态普遍增加。到出生后第21天，上述纤维的分布基本达到成年水平，尽管与表皮相关的CGRP-IR和SP-IR纤维密度明显高于成年大鼠。在所检查的任何时间点，胸部多毛皮肤均未出现以下情况：胆碱乙酰转移酶活性、乙酰胆碱酯酶组织化学染色或免疫反应性、对血管活性肠肽、胆囊收缩素或亮氨酸脑啡肽显示免疫反应性的纤维。本研究表明，发育中和成年大鼠的胸部多毛皮肤接受丰富的交感儿茶酚胺能和感觉神经支配，但不接受胆碱能神经支配。
