Poopathi Subbiah, Abidha S
Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Medical Complex, Indira Nagar, Pondicherry-60 5006, India.
J Econ Entomol. 2009 Aug;102(4):1423-30. doi: 10.1603/029.102.0404.
Chicken feathers, discarded as bioorganic wastes from poultry processing industries, are rich in keratin (structural protein). Deoiled rice bran (DRB), in contrast, is an agrowaste product consisting of protein, fiber, and carbohydrates. This study highlights a new medium for producing biopesticides from these bioorganic wastes, by culturing Bacillus sphaericus (Bs) and B. thuringiensis subsp. israelensis (Bti), to synthesize mosquitocidal toxins. Biochemical studies indicated that the amount of mosquitocidal spore/crystal toxins produced from the experimental culture medium (chicken feather waste [CFW] + DRB) is higher than that from the conventional medium (Nutrient Yeast Extract Salt Medium [NYSM]). The bacteria produced in these media (NYSM, CFW, DRB, and CFW + DRB) were bioassayed against mosquito vectors, and the toxic effect was found to be statistically similar (fiducial limits overlapping). Cost analysis based on the raw materials, indicated that, the use of CFW and DRB, as culture medium, is highly economical, for the industrial production of these mosquito pathogenic bacilli.
鸡毛作为家禽加工业的生物有机废弃物被丢弃，富含角蛋白（结构蛋白）。相比之下，脱脂米糠是一种农业废弃物产品，由蛋白质、纤维和碳水化合物组成。本研究强调了一种利用这些生物有机废弃物生产生物农药的新培养基，通过培养球形芽孢杆菌（Bs）和苏云金芽孢杆菌以色列亚种（Bti）来合成杀蚊毒素。生化研究表明，从实验培养基（鸡毛废弃物[CFW]+脱脂米糠[DRB]）中产生的杀蚊芽孢/晶体毒素量高于传统培养基（营养酵母提取物盐培养基[NYSM]）。对在这些培养基（NYSM、CFW、DRB和CFW+DRB）中产生的细菌针对蚊虫媒介进行了生物测定，发现其毒性作用在统计学上相似（置信限重叠）。基于原材料的成本分析表明，将CFW和DRB用作培养基，对于这些蚊虫致病杆菌的工业化生产而言具有很高的经济性。
