[核心蛋白聚糖对HuH7肝癌细胞体外增殖的影响及机制]
[Effects and mechanism of decorin on the proliferation of HuH7 hepatoma carcinoma cells in vitro].
作者信息
Shangguan Jian-ying, Dou Ke-feng, Li Xiao, Hu Xiao-jun, Zhang Fu-qin, Yong Zhao-sheng, Ti Zhen-yu
机构信息
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2009 Sep;25(9):780-2.
AIM
To investigate the effects and mechanism of decorin( DCN) on the proliferation of HuH7 hepatoma carcinoma cell line in vitro.
METHODS
Hepatoma carcinoma cells was cultured with DCN in different concentration (0, 25, 50, 75, 100, 125, 150, 200 microg/L) for different time(12, 24, 48, 72 h and 2 weeks). Cell activities were studied by MTT and clone test. The changes of cell cycle and apoptosis were analyzed by Flow cytometry.
RESULTS
The proliferation of HuH7 cells could be inhibited by DCN in vitro and the inhibition effect was the time and dose dependent relationship. DCN could block cell cycle at G(1); phase. Apoptosis of hepatocarcinoma cells could be efficiently induced by DCN in a time/dose-dependent manner.
CONCLUSION
DCN may be a negative regulatory protein inhibiting hepatoma carcinoma cell proliferation through inhibiting cell cycle and inducing apoptosis of cell in vitro.
目的
探讨核心蛋白聚糖（DCN）对体外培养的HuH7肝癌细胞系增殖的影响及其机制。
方法
将肝癌细胞与不同浓度（0、25、50、75、100、125、150、200μg/L）的DCN培养不同时间（12、24、48、72小时及2周）。采用MTT法和克隆试验研究细胞活性。通过流式细胞术分析细胞周期和凋亡的变化。
结果
DCN可在体外抑制HuH7细胞的增殖，且抑制作用呈时间和剂量依赖性关系。DCN可将细胞周期阻滞在G(1)期。DCN能以时间/剂量依赖性方式有效诱导肝癌细胞凋亡。
结论
DCN可能是一种负性调节蛋白，通过在体外抑制细胞周期和诱导细胞凋亡来抑制肝癌细胞的增殖。
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