Martínez Josefina, Olmos José M, Hernández José L, Pinedo Gabriel, Llorca Javier, Obregón Eduardo, Valero Carmen, González-Macías Jesús
Department of Internal Medicine, Hospital Universitario Marqués de Valdecilla, Universidad de Cantabria, RETICEF, Santander, Spain.
Clin Chim Acta. 2009 Nov;409(1-2):70-4. doi: 10.1016/j.cca.2009.08.020. Epub 2009 Sep 6.
This cross-sectional study was performed to determine the reference ranges for two bone turnover markers--aminoterminal propeptide of type I collagen (P1NP) and C-terminal telopeptide of type I collagen (beta-CrossLaps, beta-CTX)--in normal postmenopausal Spanish women as determined in serum by automated methods.
A community-based population of 1080 healthy postmenopausal women was evaluated. Data regarding risk factors for osteoporosis and fractures were collected by means of a structured questionnaire. Fasting serum levels of P1NP, beta-CTX, 25-Hydroxivitamin D (25OHD), and intact parathyroid hormone (iPTH) were measured on the Elecsys 2010 automated analyzer (Roche). BMD at lumbar spine, femoral neck and total hip was determined by DXA.
The mean age of subjects was 63+/-9. Logarithmic transformation of both markers was performed to allow for normal distributions. Mid-95% ranges for P1NP and beta-CTX were 19-100 ng/ml and 0.112-1.018 ng/ml, respectively. Mean values of P1NP (47.7+/-19.9 ng/ml) were similar to those previously determined by the manufacturer of the assays, whereas beta-CTX mean values (0.387+/-0.197 ng/ml) were lower. Both markers were higher among osteoporotic women.
Values obtained from this well-characterized population study provide reference ranges for serum automated P1NP and beta-CTX in normal Spanish postmenopausal women.
本横断面研究旨在确定通过自动化方法测定的正常绝经后西班牙女性血清中两种骨转换标志物——I型胶原氨基端前肽（P1NP）和I型胶原C端肽（β-交联C端肽，β-CTX）的参考范围。
对1080名健康绝经后女性的社区人群进行评估。通过结构化问卷收集骨质疏松症和骨折危险因素的数据。在Elecsys 2010自动分析仪（罗氏公司）上测量P1NP、β-CTX、25-羟基维生素D（25OHD）和完整甲状旁腺激素（iPTH）的空腹血清水平。通过双能X线吸收法（DXA）测定腰椎、股骨颈和全髋部的骨密度。
受试者的平均年龄为63±9岁。对两种标志物进行对数转换以使其呈正态分布。P1NP和β-CTX的95%中位数范围分别为19 - 100 ng/ml和0.112 - 1.018 ng/ml。P_INP的平均值（47.7±19.9 ng/ml）与检测试剂盒制造商先前测定的值相似，而β-CTX的平均值（0.387±0.197 ng/ml）较低。两种标志物在骨质疏松症女性中均较高。
从这项特征明确的人群研究中获得的值为正常西班牙绝经后女性血清中自动化检测的P1NP和β-CTX提供了参考范围。
