Fainaru M, Felker T E, Hamilton R L, Havel R J
Metabolism. 1977 Sep;26(9):999-1004. doi: 10.1016/0026-0495(77)90017-8.
High-density lipoproteins (HDL) (1.075 less than d less than 1.175) from perfusates of rat liver, unlike those of blood plasma, contain protein with the properties of B-apolipoprotein. This protein remains near the origin upon electrophoresis in polyacrylamide gels containing sodium dodecyl sulfate, has beta electrophoretic mobility in agarose gel, is insoluble in tetramethylurea, and precipitates with antisera to the B-apoprotein isolated from low-density lipoprotein. B-apolipoprotein in HDL from perfusates binds to concanavalin-A Sepharose and can thus be separated from the characteristic HDL, the chemical and physical properties of which are otherwise preserved. These observations suggest that in addition to the discoidal lipoproteins, another particle that contains B-apoprotein exists in HDL of perfusates.
大鼠肝脏灌注液中的高密度脂蛋白（HDL）（密度d在1.075至1.175之间），与血浆中的不同，含有具有B-载脂蛋白特性的蛋白质。这种蛋白质在含十二烷基硫酸钠的聚丙烯酰胺凝胶中电泳时停留在原点附近，在琼脂糖凝胶中具有β电泳迁移率，不溶于四甲基脲，并且能与针对从低密度脂蛋白中分离出的B-载脂蛋白的抗血清沉淀。灌注液HDL中的B-载脂蛋白与伴刀豆球蛋白A琼脂糖结合，因此可以与特征性HDL分离，而后者的化学和物理性质得以保留。这些观察结果表明，除了盘状脂蛋白外，灌注液HDL中还存在另一种含有B-载脂蛋白的颗粒。
