Mishra Shrutkirti, Singh Varsha, Rao G R Koteswar, Jain Ashok Kumar, Dixit Vinod Kumar, Gulati Anil Kumar, Nath Gopal
Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005, India.
J Infect Dev Ctries. 2008 Jun 1;2(3):206-10. doi: 10.3855/jidc.264.
Efficacy of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and stool PCR was evaluated, before and after treatment, in a country with a high prevalence of H. pylori infection.
A total of 52 patients with dyspeptic symptoms were included in the study. Antral biopsy was collected during pre- and post-therapy periods for rapid urease test (RUT) and PCR. Similarly stool specimens for PCR and HpSA test were collected during both the periods from all 52 patients. Biopsy, PCR and RUT results together were considered the "gold standard."
On the basis of gold standard tests, 40/52 patients were H. pylori positive. The sensitivity and specificity of HpSA test were 80% and 83.3% respectively in untreated patients. On the other hand, the sensitivity and specificity of stool PCR in untreated patients were 72.5% and 100% respectively. After eradication therapy, the results of both RUT and biopsy PCR were negative in 87.5% and positive in 12.5% of the patients. Although post treatment sensitivity of HpSA and stool PCR was equal (60%), specificity of HpSA and stool PCR were 68.6% and 97.1% respectively.
The H. pylori stool tests represent a non-invasive concept for diagnosis of infection. Both HpSA and stool PCR seem to be satisfactory tests for pre-eradication as well as assessment of infection. But stool PCR is a better indicator than HpSA test in the post-eradication assessment of infection.
在幽门螺杆菌（H. pylori）感染率较高的国家，对幽门螺杆菌粪便抗原酶免疫测定（HpSA）和粪便聚合酶链反应（PCR）在治疗前后的效果进行了评估。
共有52例有消化不良症状的患者纳入本研究。在治疗前和治疗后采集胃窦活检组织进行快速尿素酶试验（RUT）和PCR检测。同样，在这两个时期从所有52例患者中采集粪便标本进行PCR和HpSA检测。活检、PCR和RUT结果共同被视为“金标准”。
根据金标准检测，52例患者中有40例幽门螺杆菌呈阳性。在未治疗的患者中，HpSA检测的敏感性和特异性分别为80%和83.3%。另一方面，未治疗患者粪便PCR的敏感性和特异性分别为72.5%和100%。根除治疗后，RUT和活检PCR结果在87.5%的患者中为阴性，在12.5%的患者中为阳性。虽然治疗后HpSA和粪便PCR的敏感性相同（60%），但HpSA和粪便PCR的特异性分别为68.6%和97.1%。
幽门螺杆菌粪便检测是一种用于诊断感染的非侵入性方法。HpSA和粪便PCR似乎都是用于根除前诊断以及评估感染的满意检测方法。但在根除后感染评估中，粪便PCR比HpSA检测是更好的指标。
