Zerek-Mełeń G, Lewiński A, Szkudliński M
Department of Experimental Endocrinology and Hormone Diagnostics, Medical Academy of Lódź, Poland.
Regul Pept. 1990 May 21;28(3):293-300. doi: 10.1016/0167-0115(90)90027-t.
The aim of the present study has been to examine the effects of various concentrations of somatostatin (SS), epidermal growth factor (EGF), as well as of interactions among SS, EGF and thyrotropin (TSH) in their influence upon the mitotic activity of thyroid follicular cells (TFC) in organ culture. The stathmokinetic method was employed. It was shown that: (1) SS, at the concentration of 10(-7) M, suppressed the mitogenic effect of TSH, as well as of TSH and EGF employed together, on TFC; (2) EGF, at the concentration of 10 and 100 ng/ml, increased the mean mitotic activity rate of TFC; (3) TSH and EGF revealed an additive action on TFC proliferation. The obtained results evidently suggest an antiproliferative effect of SS and mitogenic action of EGF on TFC in organ culture.
本研究的目的是检测不同浓度的生长抑素（SS）、表皮生长因子（EGF），以及SS、EGF和促甲状腺激素（TSH）之间的相互作用对器官培养中甲状腺滤泡细胞（TFC）有丝分裂活性的影响。采用了有丝分裂抑制法。结果表明：（1）浓度为10^(-7) M的SS可抑制TSH以及TSH与EGF共同作用对TFC的促有丝分裂作用；（2）浓度为10和100 ng/ml的EGF可提高TFC的平均有丝分裂活性率；（3）TSH和EGF对TFC增殖表现出相加作用。所得结果明显提示，在器官培养中SS对TFC有抗增殖作用，而EGF对TFC有促有丝分裂作用。
