成纤维细胞与肿瘤细胞相互作用增强人胃硬癌细胞系（KATO-III）中的黏液积累

Enhancement of mucus accumulation in a human gastric scirrhous carcinoma cell line (KATO-III) by fibroblast-tumor cell interaction.

作者信息

Yamamoto R, Iishi H, Tatsuta M, Nakamura H, Terada N, Komatsu K, Matsusaka T

机构信息

Department of Gastrointestinal Oncology, Center for Adult Diseases, Osaka, Japan.

出版信息

Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;59(1):26-31. doi: 10.1007/BF02899383.

DOI:10.1007/BF02899383

PMID:1974095

Abstract

Human fibroblasts (WI-38 cells) were found to enhance mucus accumulation by human scirrhous carcinoma cells (KATO-III cells). Coculture of KATO-III with WI-38 cells resulted in enlargement of the KATO-III cells and increases in the proportions of PAS- and colloidal iron-positive KATO-III cells. These morphological alterations were reversed when the KATO-III cells were again cultured without WI-38 cells. Conditioned media from cultures of WI-38 cells or cocultures of KATO-III and WI-38 cells induced the same morphological alterations in KATO-III cells, suggesting that WI-38 cells produce a factor or factors that enhance mucus accumulation in KATO-III cells. This factor seemed to be a protein with a molecular weight of more than 10,000 daltons.

摘要

研究发现，人成纤维细胞（WI-38细胞）可增强人硬癌细胞（KATO-III细胞）的黏液积累。KATO-III细胞与WI-38细胞共培养导致KATO-III细胞增大，且PAS和胶体铁阳性的KATO-III细胞比例增加。当KATO-III细胞再次在无WI-38细胞的条件下培养时，这些形态学改变得以逆转。WI-38细胞培养的条件培养基或KATO-III与WI-38细胞的共培养物在KATO-III细胞中诱导出相同的形态学改变，这表明WI-38细胞产生一种或多种可增强KATO-III细胞黏液积累的因子。该因子似乎是一种分子量超过10,000道尔顿的蛋白质。