Gay N J
Department of Biochemistry, University of Cambridge, U.K.
Biochim Biophys Acta. 1990 Jul 30;1049(3):249-54. doi: 10.1016/0167-4781(90)90094-i.
A polyclonal antibody has been prepared that specifically recognises a nuclear protein antigen in Drosophila embryos. During development, the antigen appears initially to be uniformly distributed but by nuclear division cycle 10 is seen to accumulate in nuclei in a manner suggesting that it is destroyed or becomes modified upon transition from S- to M phase of the nuclear division cycle. This conclusion is supported by the observed disappearance of the antigen from the postblastoderm nuclei in a manner that reflects the pattern of the first asynchronous postblastoderm cell division and persistence in the polyploid nuclei of the amnioserosa that do not undergo further cell or nuclear division. In Western blot experiments, the antibody detects specifically a 105 kDa nuclear protein that probably corresponds to the antigen detected in embryos by immunocytochemical means.
已经制备了一种多克隆抗体，它能特异性识别果蝇胚胎中的一种核蛋白抗原。在发育过程中，该抗原最初似乎均匀分布，但到核分裂周期10时，可观察到它以一种表明其在从核分裂周期的S期过渡到M期时被破坏或发生修饰的方式在细胞核中积累。这一结论得到了以下观察结果的支持：抗原从胚盘后细胞核中消失的方式反映了胚盘后第一次异步细胞分裂的模式，并且在不进行进一步细胞或核分裂的羊膜浆膜多倍体细胞核中持续存在。在蛋白质印迹实验中，该抗体特异性检测到一种105 kDa的核蛋白，它可能与通过免疫细胞化学方法在胚胎中检测到的抗原相对应。
