Bier E, Ackerman L, Barbel S, Jan L, Jan Y N
Howard Hughes Medical Institute, University of California, San Francisco 94143.
Science. 1988 May 13;240(4854):913-6. doi: 10.1126/science.3129785.
An antigen found only in neuronal nuclei of Drosophila melanogaster is revealed by staining with a monoclonal antibody (Mab44C11). This antigen appears early in development, before neurons show any other signs of antigenic or morphologic differentiation, and persists throughout development. This nuclear staining permits reliable detection of neurons in developmental studies of wild-type and mutant flies. Protein immunoblot analyses and immune precipitation experiments show that the neuronal nuclear antigen is a 50-kilodalton polypeptide.
用单克隆抗体（Mab44C11）染色可揭示一种仅在黑腹果蝇神经元细胞核中发现的抗原。这种抗原在发育早期出现，早于神经元表现出任何其他抗原或形态分化的迹象，并在整个发育过程中持续存在。这种细胞核染色能够在野生型和突变型果蝇的发育研究中可靠地检测神经元。蛋白质免疫印迹分析和免疫沉淀实验表明，神经元核抗原是一种50千道尔顿的多肽。
