Department of Parasitology, Faculty of Veterinary Medicine, Firat University, 23119 Elazig, Turkey.
Trans R Soc Trop Med Hyg. 2010 Jan;104(1):10-5. doi: 10.1016/j.trstmh.2009.07.025. Epub 2009 Sep 10.
This study investigated the presence of the pathogen Anaplasma phagocytophilum in ixodid ticks removed from humans living in three provinces (Giresun, Trabzon, Rize) in the east of the Black Sea Region of Turkey. A total of 1097 ixodid ticks were examined for the presence of A. phagocytophilum DNA. From the 95 pooled tick samples tested, species-specific fragments of A. phagocytophilum (11/95 samples, 11.6%) were amplified by nested PCR. Adult Ixodes ricinus (9/53 samples, 17.0%) and Ixodes spp. nymphs (2/9 samples, 22.2%) were infected with A. phagocytophilum. None of the remaining tick species gave a positive result for the presence of the pathogen. All nested PCR-positive samples were directly sequenced. The partial sequences (457bp) of the amplicons obtained from the infected tick pools were 100% identical to one another and to previously isolated sequences from human patients. To obtain a longer 16S rRNA gene sequence, one representative sample was reamplified with the universal primer set. The longer representative sequence (1306bp) also shared 99.92% similarity (a single adenine deletion) with the recently reported complete sequence of A. phagocytophilum.
本研究调查了在土耳其黑海地区东部的三个省份（吉雷松、特拉布宗和里泽）生活的人群身上采集的蜱虫中是否存在病原体嗜吞噬细胞无形体。共检查了 1097 只蜱虫以检测 A. phagocytophilum DNA 的存在。通过巢式 PCR 扩增，从 95 个混合蜱样本中检测到了 A. phagocytophilum 的种特异性片段（11/95 样本，11.6%）。感染 A. phagocytophilum 的成虫硬蜱（9/53 样本，17.0%）和蜱若虫（2/9 样本，22.2%）。其余的蜱种均未检测到病原体的存在。所有巢式 PCR 阳性样本均直接测序。从感染的蜱虫样本中获得的扩增子的部分序列（457bp）彼此之间以及与先前从人类患者中分离的序列完全相同。为了获得更长的 16S rRNA 基因序列，用通用引物对一个代表性样本进行了再扩增。较长的代表性序列（1306bp）与最近报道的 A. phagocytophilum 完整序列也共享 99.92%的相似性（单个腺嘌呤缺失）。
