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土耳其小型反刍动物中[具体内容缺失]及其相关变体的分子检测与系统发育分析

Molecular Detection and Phylogeny of and Related Variants in Small Ruminants from Turkey.

作者信息

Aktaş Münir, Özübek Sezayi, Uluçeşme Mehmet Can

机构信息

Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey.

出版信息

Animals (Basel). 2021 Mar 14;11(3):814. doi: 10.3390/ani11030814.

DOI:10.3390/ani11030814
PMID:33799376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8001643/
Abstract

causes tick-borne fever in small ruminants. Recently, novel variants related to have been reported in ruminants from Tunisia, Italy, South Korea, Japan, and China. Based on and genes and sequencing, we screened the frequency of and related variants in 433 apparently healthy small ruminants in Turkey. spp. overall infection rates were 27.9% (121/433 analyzed samples). The frequency of and -like 1 infections was 1.4% and 26.5%, respectively. No -like 2 was detected in the tested animals. The prevalence of spp. was comparable in species, and no significant difference was detected between sheep and goats, whereas the prevalence significantly increased with tick infestation. Sequencing confirmed PCR-RFLP data and showed the presence of and -like-1 variant in the sampled animals. Phylogeny-based on gene revealed the -like 1 in a separate clade together with the previous isolates detected in small ruminants and ticks. In this work, -like 1 has been detected for the first time in sheep and goats from Turkey. This finding revealed that the variant should be considered in the diagnosis of caprine and ovine anaplasmosis.

摘要

引起小反刍兽蜱传发热。最近,在来自突尼斯、意大利、韩国、日本和中国的反刍动物中报告了与[相关内容缺失]有关的新型变体。基于[相关基因缺失]基因和测序,我们在土耳其433只表面健康的小反刍动物中筛查了[相关内容缺失]及相关变体的频率。[相关病原体缺失]属的总体感染率为27.9%(121/433份分析样本)。[相关病原体缺失]及类似[病原体相关内容缺失]1的感染频率分别为1.4%和26.5%。在受试动物中未检测到类似[病原体相关内容缺失]2。[相关病原体缺失]属的流行率在不同物种中相当,绵羊和山羊之间未检测到显著差异,而随着蜱虫感染,流行率显著增加。测序证实了PCR-RFLP数据,并显示在采样动物中存在[相关病原体缺失]及类似[病原体相关内容缺失]1变体。基于[相关基因缺失]基因的系统发育分析显示,类似[病原体相关内容缺失]1与先前在小反刍动物和蜱虫中检测到的分离株一起位于一个单独的进化枝中。在这项工作中,首次在土耳其的绵羊和山羊中检测到类似[病原体相关内容缺失]1。这一发现表明,在诊断山羊和绵羊无形体病时应考虑该变体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bca/8001643/f4fc37c59ef6/animals-11-00814-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bca/8001643/23e19aa07c4d/animals-11-00814-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bca/8001643/f4fc37c59ef6/animals-11-00814-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bca/8001643/23e19aa07c4d/animals-11-00814-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0bca/8001643/f4fc37c59ef6/animals-11-00814-g002.jpg

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