Chalmers A H, Hare C, Woolley G, Frazer I H
Department of Pathology, Mater Misericordiae Public Hospital, South Brisbane, Australia.
Immunol Cell Biol. 1990 Apr;68 ( Pt 2):81-5. doi: 10.1038/icb.1990.12.
We measured two ectoenzymes, ecto-5'-nucleotidase (NT) and dipeptidyl peptidase IV (DP) in the peripheral blood lymphocytes of various groups of HIV-infected patients because of the previous implied relationship of these enzymes to immune function. NT expressed as mean nmol/h per mg protein (+/- s.d.) was significantly depressed in the HIV-seropositive asymptomatic (42 +/- 32; P less than 0.01) and AIDS groups (14 +/- 7; P less than 0.002) when compared with a healthy HIV-seronegative male population (83 +/- 27). The NT activities in asymptomatic HIV-seropositive and HIV-seronegative high risk groups (53 +/- 30) were not significantly different from one another but both groups had significantly higher enzyme activities than the AIDS group (P = 0.01 and less than 0.002, respectively). The seronegative high risk and normal healthy group had similar NT activities. DP activities expressed as mean nmol/h per mg protein (+/- s.d.) in both seropositive asymptomatic (0.188 +/- 0.038) and high risk seronegative (0.180 +/- 0.05) groups had higher enzyme activities than the healthy seronegative (0.117 +/- 0.015; P = 0.02 and 0.05, respectively) and AIDS group (0.096 +/- 0.036; P = 0.002 and 0.02, respectively). The healthy seronegative group had DP activities not significantly different to the AIDS groups. Similarly the high risk seronegative and healthy seropositive group had similar DP activities. These results taken together indicate that measurement of both DP and NT should be evaluated prospectively as a monitor of the clinical progression of HIV infection.
由于先前暗示这些酶与免疫功能有关，我们检测了不同组HIV感染患者外周血淋巴细胞中的两种外切酶，即外切5'-核苷酸酶（NT）和二肽基肽酶IV（DP）。与健康的HIV血清阴性男性人群（83±27）相比，HIV血清阳性无症状组（42±32；P<0.01）和艾滋病组（14±7；P<0.002）中以每毫克蛋白每小时平均纳摩尔数（±标准差）表示的NT显著降低。无症状HIV血清阳性组和HIV血清阴性高危组的NT活性（53±30）彼此无显著差异，但两组的酶活性均显著高于艾滋病组（分别为P=0.01和<0.002）。血清阴性高危组和正常健康组的NT活性相似。在血清阳性无症状组（0.188±0.038）和高危血清阴性组（0.180±0.05）中，以每毫克蛋白每小时平均纳摩尔数（±标准差）表示的DP活性均高于健康血清阴性组（0.117±0.015；分别为P=0.02和0.05）和艾滋病组（0.096±0.036；分别为P=0.002和0.02）。健康血清阴性组的DP活性与艾滋病组无显著差异。同样，高危血清阴性组和健康血清阳性组的DP活性相似。综合这些结果表明，应前瞻性地评估DP和NT的检测，作为HIV感染临床进展的监测指标。
