Agostini C, Semenzato G, Vinante F, Sinicco A, Trentin L, Zambello R, Zuppini B, Zanotti R, Siviero F, Veneri D
Department of Clinical Medicine, Padua University, Italy.
Clin Immunol Immunopathol. 1989 Jan;50(1 Pt 1):146-53. doi: 10.1016/0090-1229(89)90229-8.
In this study we investigated the serological levels of the soluble form of the CD8 molecule (s-CD8) in 97 human immunodeficiency virus (HIV) seropositive patients. The control groups included 20 normal heterosexual subjects and 19 healthy seronegative subjects belonging to risk groups for AIDS. Our results show that patients with HIV infection have significantly higher levels of s-CD8 U/ml than the control groups. When the patients were further subdivided according to the Centers for Disease Control (CDC) classification, s-CD8 U/ml values were consistently increased in all HIV patients, irrespective of the CDC stages. No statistically significant correlation was found between the serological levels of s-CD8/ml and the absolute numbers of CD8 lymphocytes/mm3, in both HIV seropositive patients and control groups. Since in the more advanced stages of HIV infection (IV-A, IV-C1) the decrease in the absolute number of CD8+ cells was not followed by a decrease in s-CD8 levels, it is conceivable that an increased release and/or shedding of s-CD8 per cell might occur in these patients. In fact, when the results were expressed as s-CD8 units per CD8 positive cell (s-CD8/absolute number of CD8), the levels of s-CD8/cell were higher in patients belonging to the IV-A and IV-C1 CDC groups (1.94 U/cell +/- 0.33 and 3.39 U/cell +/- 0.5, respectively) compared to normal controls (P less than 0.001), HIV seronegative subjects at risk for AIDS (P less than 0.001), and the other patients' groups (II and III CDC groups, respectively, P less than 0.001 and P less than 0.001). The evidence herein provided that in patients with HIV infection s-CD8 levels are increased suggests a possible pathogenetic role of the cells involved in the release of this molecule.
在本研究中，我们调查了97例人类免疫缺陷病毒（HIV）血清学阳性患者中可溶性CD8分子（s-CD8）的血清学水平。对照组包括20名正常异性恋者和19名属于艾滋病风险组的健康血清学阴性者。我们的结果显示，HIV感染患者的s-CD8 U/ml水平显著高于对照组。当根据疾病控制中心（CDC）分类对患者进一步细分时，所有HIV患者的s-CD8 U/ml值均持续升高，与CDC分期无关。在HIV血清学阳性患者和对照组中，s-CD8/ml的血清学水平与CD8淋巴细胞/mm³的绝对数量之间均未发现统计学上的显著相关性。由于在HIV感染的更晚期阶段（IV-A、IV-C1），CD8⁺细胞绝对数量的减少并未伴随s-CD8水平的降低，因此可以推测这些患者可能每细胞s-CD8的释放和/或脱落增加。事实上，当结果表示为每CD8阳性细胞的s-CD8单位（s-CD8/CD8绝对数量）时，与正常对照组（P<0.001）、有艾滋病风险的HIV血清学阴性者（P<0.001）以及其他患者组（分别为II和III CDC组，P<0.001和P<0.001）相比，属于IV-A和IV-C1 CDC组的患者的s-CD8/细胞水平更高（分别为1.94 U/细胞±0.33和3.39 U/细胞±0.5）。本文提供的证据表明，HIV感染患者的s-CD8水平升高，提示参与该分子释放的细胞可能具有致病作用。
