Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei, Taiwan.
Res Vet Sci. 2010 Apr;88(2):285-93. doi: 10.1016/j.rvsc.2009.08.006. Epub 2009 Sep 11.
Three canine mammary tumor (CMT) cell lines, namely DE-E, DE-F and DE-SF, have been established from a surgically excised specimen of a malignant mammary tumor. These CMT cell lines have been cultured for over 200 passages. The cell doubling time was estimated to be approximately 30 h for all three cell lines. DE-E, DE-F and DE-SF were epithelial, fibroblast and spindle fibroblast in morphology, respectively. Under electron microscope, DE-F and DE-SF cells displayed a higher nucleus/cytoplasm ratio as compared with DE-E. Variation in chromosome number was also observed in the three cell lines. In addition to the morphological characteristics, these cell lines displayed differential patterns of several known mammary tumor cell markers. Following xenotransplantation of the CMT cells into nude mice, DE-F and DE-SF developed tumors within 2 weeks, whereas DE-E failed to develop any visible tumor up to 8 weeks after injection. Lastly, the CMT cell lines exhibited differential chemoresistance to several anti-tumor drugs, including melatonin, cyclosporine A, tamoxifen and indole, suggesting that these cell lines can be used as a comparative experimental model for the tumorigenesis of mammary carcinomas and a valuable tool for anti-cancer drug screening.
从手术切除的恶性乳腺肿瘤标本中建立了三种犬乳腺肿瘤 (CMT) 细胞系，分别为 DE-E、DE-F 和 DE-SF。这些 CMT 细胞系已经培养了超过 200 代。三种细胞系的细胞倍增时间估计约为 30 小时。DE-E、DE-F 和 DE-SF 在形态上分别为上皮细胞、成纤维细胞和纺锤形成纤维细胞。在电子显微镜下，与 DE-E 相比，DE-F 和 DE-SF 细胞显示出更高的核/细胞质比。三种细胞系也观察到染色体数目的变化。除了形态特征外，这些细胞系还显示出几种已知的乳腺肿瘤细胞标志物的不同表达模式。将 CMT 细胞异种移植到裸鼠中后，DE-F 和 DE-SF 在 2 周内形成肿瘤，而 DE-E 在注射后 8 周内未形成任何可见肿瘤。最后，CMT 细胞系对几种抗肿瘤药物，包括褪黑素、环孢素 A、他莫昔芬和吲哚，表现出不同的耐药性，表明这些细胞系可用作乳腺癌发生的比较实验模型，也是抗癌药物筛选的有价值工具。
