BMC Vet Res. 2014 Sep 30;10:229. doi: 10.1186/s12917-014-0229-0.
Canine mammary tumors (CMTs) are the most common type of cancer found in female dogs. Establishment and evaluation of tumor cell lines can facilitate investigations of the biological mechanisms of cancer. Different cell models are used to investigate genetic, epigenetic, and cellular pathways, cancer progression, and cancer therapeutics. Establishment of new cell models will greatly facilitate research in this field. In the present study, we established and characterized two new CMT cell lines derived from a single CMT.
We established two cell lines from a single malignant CMT specimen: DTK-E and DTK-SME. Morphologically, the DTK-E cells were large, flat, and epithelial-like, whereas DTK-SME cells were round and epithelial-like. Doubling times were 24 h for DTK-E and 18 h for DTK-SME. On western blots, both cell lines expressed cytokeratin AE1, vimentin, cytokeratin 7 (CK7), and heat shock protein 27 (HSP27). Moreover, investigation of chemoresistance revealed that DTK-SME was more resistant to doxorubicin-induced apoptosis than DTK-E was. After xenotransplantation, both DTK-E and DTK-SME tumors appeared within 14 days, but the average size of DTK-SME tumors was greater than that of DTK-E tumors after 56 days.
We established two new cell lines from a single CMT, which exhibit significant diversity in cell morphology, protein marker expression, tumorigenicity, and chemoresistance. The results of this study revealed that the DTK-SME cell line was more resistant to doxorubicin-induced apoptosis and exhibited higher tumorigenicity in vivo than the DTK-E cell line. We anticipate that the two novel CMT cell lines established in this study will be useful for investigating the tumorigenesis of mammary carcinomas and for screening anticancer drugs.
犬乳腺肿瘤（CMTs）是女性犬中最常见的癌症类型。建立和评估肿瘤细胞系可以促进对癌症生物学机制的研究。不同的细胞模型用于研究遗传、表观遗传和细胞途径、癌症进展和癌症治疗学。建立新的细胞模型将极大地促进该领域的研究。在本研究中，我们从单个 CMT 中建立并鉴定了两种新的 CMT 细胞系。
我们从单个恶性 CMT 标本中建立了两种细胞系：DTK-E 和 DTK-SME。形态上，DTK-E 细胞大而扁平，呈上皮样，而 DTK-SME 细胞呈圆形，呈上皮样。DTK-E 的倍增时间为 24 小时，DTK-SME 的倍增时间为 18 小时。在 Western blot 上，两种细胞系均表达细胞角蛋白 AE1、波形蛋白、细胞角蛋白 7（CK7）和热休克蛋白 27（HSP27）。此外，对化疗耐药性的研究表明，DTK-SME 对阿霉素诱导的细胞凋亡的耐药性高于 DTK-E。异种移植后，DTK-E 和 DTK-SME 肿瘤均在 14 天内出现，但 56 天后 DTK-SME 肿瘤的平均大小大于 DTK-E 肿瘤。
我们从单个 CMT 中建立了两种新的细胞系，它们在细胞形态、蛋白标志物表达、致瘤性和化疗耐药性方面表现出显著的多样性。本研究的结果表明，与 DTK-E 细胞系相比，DTK-SME 细胞系对阿霉素诱导的细胞凋亡的耐药性更高，体内致瘤性更强。我们预计本研究中建立的两种新型 CMT 细胞系将有助于研究乳腺癌的发生机制，并用于筛选抗癌药物。
