Minarini Luciene A R, Poirel Laurent, Trevisani Nathalia A C, Darini Ana Lucia C, Nordmann Patrice
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris-Sud, K.-Bicêtre, France.
Diagn Microbiol Infect Dis. 2009 Oct;65(2):202-6. doi: 10.1016/j.diagmicrobio.2009.05.021.
Two hundred fifty-seven nalidixic acid-resistant enterobacterial isolates were collected in a Brazilian community from January 2000 to May 2005 to determine the prevalence of plasmid-encoded extended-spectrum beta-lactamases. The bla(CTX-M) genetic environment was determined by polymerase chain reaction and sequencing. Eleven isolates (4.2%) harbored a bla(CTX-M-2) gene, 3 isolates bla(CTX-M-9), 2 isolates bla(CTX-M-8), and 6 isolates bla(SHV-5). Two novel bla(CTX-M-2) variants, namely, bla(CTX-M-74) and bla(CTX-M-75), were identified.
2000年1月至2005年5月期间，在巴西一个社区收集了257株耐萘啶酸肠杆菌分离株，以确定质粒编码的超广谱β-内酰胺酶的流行情况。通过聚合酶链反应和测序确定bla(CTX-M)基因环境。11株分离株（4.2%）携带bla(CTX-M-2)基因，3株携带bla(CTX-M-9)，2株携带bla(CTX-M-8)，6株携带bla(SHV-5)。鉴定出两种新型bla(CTX-M-2)变体，即bla(CTX-M-74)和bla(CTX-M-75)。
