Institute for Molecules and Materials, Department of Biophysical Chemistry, Radboud University Nijmegen, 6525 AJ Nijmegen, The Netherlands.
Anal Biochem. 2010 Jan 15;396(2):280-3. doi: 10.1016/j.ab.2009.09.013. Epub 2009 Sep 11.
S-Adenosyl-L-methionine (SAM) is the preferred cofactor for biological methyl group transfers to various substrates such as nucleic acids, proteins, and lipids. Here we present stereospecific (>95% of the desired enantiomer) and high-yield preparation of four fluorescent and biologically active SAM analogs and demonstrate their usefulness in binding studies. Using a fluorescence titration experiment, we obtained a K(d) of 0.38 microM for the S-2,6-diaminopurinylmethionine-SAM-III riboswitch complex.
S-腺苷-L-蛋氨酸（SAM）是生物甲基转移到各种底物（如核酸、蛋白质和脂质）的首选辅因子。在这里，我们呈现了立体特异性（所需对映体的>95%）和高产制备四种荧光和生物活性 SAM 类似物，并证明了它们在结合研究中的有用性。通过荧光滴定实验，我们得到了 S-2,6-二氨基嘌呤基蛋氨酸-SAM-III 核糖开关复合物的 K(d)为 0.38 microM。
