Department of Chemistry, Razi University, Kermanshah, Iran.
Food Chem Toxicol. 2010 Jan;48(1):24-8. doi: 10.1016/j.fct.2009.09.007. Epub 2009 Sep 11.
This study is outlined to probe the chemical composition of essential oil and in vitro antioxidant activity of the essential oil and methanol extracts of Psammogeton canescens. The chemical composition of the hydrodistilled essential oil of the aerial parts of P. canescens was analyzed by GC and GC/MS. The main constituents of the oil were found to be beta-bisabolene (33.35%), apiole (28.34%), alpha-pinene (11.86%) and dill apiole (8.17%). Antioxidant activities of the samples were determined by three various testing systems namely DPPH, beta-carotene/linoleic acid, and reducing power assay. In DPPH system, the highest radical-scavenging activity was seen by the polar subfraction of methanol extract (49.5+/-1.21mug/ml). Furthermore, in the second case the inhibition capacity (%) of the polar subfraction (92.40%+/-0.72) found to be the stronger one. However, in the reducing power assay, a reverse activity pattern more than in the first two systems was observed, and the essential oil was stronger radical reducer than was the methanol extract in all of the concentration tested. Our findings demonstrate that the essential oil and methanol extracts of P. canescens possess significant antioxidant activities and may be suggested as a new potential source of natural antioxidant.
本研究旨在探究灯心草属植物地上部分精油的化学成分和体外抗氧化活性及其甲醇提取物的抗氧化活性。采用 GC 和 GC/MS 对水蒸汽蒸馏法提取的精油进行化学成分分析。结果表明,该油的主要成分为 β- 倍半水芹烯(33.35%)、阿魏酸(28.34%)、α- 蒎烯(11.86%)和大茴香脑(8.17%)。采用三种不同的测试系统(DPPH、β- 胡萝卜素/亚油酸和还原力测定法)测定了样品的抗氧化活性。在 DPPH 体系中,甲醇提取物的极性部分表现出最强的自由基清除活性(49.5+/-1.21mug/ml)。此外,在第二种情况下,极性部分的抑制能力(92.40%+/-0.72)最强。然而,在还原力测定中,与前两种体系相比,观察到相反的活性模式,并且在所有测试浓度下,精油的自由基还原能力均强于甲醇提取物。我们的研究结果表明,灯心草属植物的精油和甲醇提取物具有显著的抗氧化活性,可作为天然抗氧化剂的新潜在来源。