从分离的细胞核中分离出的新合成的多瘤病毒DNA短片段的滞留现象。
Strandedness of newly synthesized short pieces of polyoma DNA from isolated nuclei.
作者信息
Flory P J
出版信息
Nucleic Acids Res. 1977;4(5):1449-64. doi: 10.1093/nar/4.5.1449.
Abstract
The discontinuous synthesis of the complementary strands of polyoma DNA in isolated nuclei has been studied by hybridization techniques. The relative amounts of the newly synthesized complementary strands were compared by separately annealing them to denatured HpaII restriction fragments. In every case an excess (1.4- to 2.4-fold) of short pieces of the strand growing in the 3' leads to 5' direction was found.
摘要
利用杂交技术研究了多瘤病毒DNA互补链在分离细胞核中的不连续合成。通过将新合成的互补链分别与变性的HpaII限制性片段退火，比较了它们的相对量。在每种情况下，都发现3'到5'方向生长的链的短片段过量（1.4至2.4倍）。
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