多瘤病毒复制过程中与RNA相连的短DNA片段。
RNA-linked short DNA fragments during polyoma replication.
作者信息
Magnusson G, Pigiet V, Winnacker E L, Abrams R, Reichard P
出版信息
Proc Natl Acad Sci U S A. 1973 Feb;70(2):412-5. doi: 10.1073/pnas.70.2.412.
Abstract
During in vitro incubation, nuclei from polyoma-infected cells elongate the daughter strands of the replicative intermediate of polyoma DNA. This process is now shown to involve the transient formation of short fragments (4-5 S), a process that is stimulated by the addition of ribonucleoside triphosphates. The presence of stretches of RNA at the 5'-end of short DNA chains was determined from Cs(2)SO(4) equilibrium centrifugation and from the finding that isotope from alpha-(32)P-labeled deoxynucleoside triphosphates was recovered in 2'(3')-ribonucleotides after alkaline hydrolysis. Transfer occurred preferentially with [alpha-(32)P]dCTP as substrate. Starvation for deoxynucleotides by in vivo treatment with hydroxyurea resulted in the accumulation of short fragments that are deficient in RNA. Our results suggest that a late step during the discontinuous synthesis of polyoma DNA is selectively inhibited when deoxynucleotides are in short supply.
摘要
在体外培养过程中，来自多瘤病毒感染细胞的细胞核会延长多瘤病毒DNA复制中间体的子链。现在表明这个过程涉及短片段（4 - 5S）的短暂形成，这一过程会因添加核糖核苷三磷酸而受到刺激。通过Cs₂SO₄平衡离心以及发现α - ³²P标记的脱氧核苷三磷酸中的同位素在碱性水解后在2'（3'） - 核糖核苷酸中回收，确定了短DNA链5'端存在RNA片段。以[α - ³²P]dCTP作为底物时，转移优先发生。用羟基脲进行体内处理使脱氧核苷酸饥饿，导致缺乏RNA的短片段积累。我们的结果表明，当脱氧核苷酸供应不足时，多瘤病毒DNA不连续合成过程中的后期步骤会受到选择性抑制。
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RNA-linked short DNA fragments during polyoma replication.多瘤病毒复制过程中与RNA相连的短DNA片段。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):412-5. doi: 10.1073/pnas.70.2.412.
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