Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 186-8650 Tokyo, Japan.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1788-1793. doi: 10.1099/ijs.0.015958-0. Epub 2009 Sep 11.
A novel strictly anaerobic, non-spore-forming, non-motile, non-flagellated, rod-shaped, Gram-negative bacterium (YIT 12066T) was isolated from human faeces. The isolate was negative for catalase, oxidase, urease, hydrolysis of aesculin and gelatin, nitrate reduction and indole production. The major end products of glucose metabolism were succinate and acetate. The major cellular fatty acids (>10%) were C14:0, C18:1omega7c, C18:1omega9c, C16:1omega7c and C16:0. The G+C content of the DNA was 40.3 mol%. 16S rRNA gene sequence analysis showed that strain YIT 12066T was most closely related to members of the family Succinivibrionaceae, with sequence similarity of 92-87%. However, some phenotypic characteristics such as cellular morphology and the major fatty acid profile of strain YIT 12066T were markedly different from those of other members of the family Succinivibrionaceae. On the basis of both phylogenetic and phenotypic evidence, it is suggested that strain YIT 12066T represents a novel species in a new genus, for which the name Succinatimonas hippei gen. nov., sp. nov. is proposed; the type strain of Succinatimonas hippei is YIT 12066T (=DSM 22608T =JCM 16073T).
从人类粪便中分离到一株新型严格厌氧菌，不产芽孢、不运动、不具鞭毛、杆状、革兰氏阴性细菌（YIT 12066T）。该分离物过氧化氢酶、氧化酶、脲酶、七叶灵和明胶水解、硝酸盐还原和吲哚产生均为阴性。葡萄糖代谢的主要终产物为琥珀酸和乙酸。主要细胞脂肪酸（>10%）为 C14:0、C18:1ω7c、C18:1ω9c、C16:1ω7c 和 C16:0。DNA 的 G+C 含量为 40.3 mol%。16S rRNA 基因序列分析表明，菌株 YIT 12066T 与琥珀酸单胞菌科的成员最为密切相关，序列相似性为 92-87%。然而，菌株 YIT 12066T 的一些表型特征，如细胞形态和主要脂肪酸图谱，与琥珀酸单胞菌科的其他成员明显不同。基于系统发育和表型证据，建议菌株 YIT 12066T 代表一个新属中的一个新种，命名为 Hippea succinatimonas gen. nov.，sp. nov.；Hippea succinatimonas 的模式株为 YIT 12066T（=DSM 22608T =JCM 16073T）。
