Morotomi Masami, Nagai Fumiko, Sakon Hiroshi, Tanaka Ryuichiro
Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2716-20. doi: 10.1099/ijs.0.2008/000810-0.
Two anaerobic, non-spore-forming, bacteria (YIT 11850(T) and YIT 11860(T)) that stained Gram-negative, were isolated from human faeces. Cells of strain YIT 11850(T) were coccobacilli, asaccharolytic and largely unreactive, with only traces of lactate and propionate as metabolic end products; however, strain YIT 11850(T) was able to decarboxylate succinate to propionate. The DNA G+C content of strain YIT 11850(T) was 51.9 mol%. Following 16S rRNA gene sequence analysis, this strain was found to be most closely related to Dialister propionicifaciens, with 95.1 % sequence similarity between the two taxa. Biochemical data supported the affiliation of strain YIT 11850(T) to the genus Dialister. Strain YIT 11850(T) therefore represents a novel species for which the name Dialister succinatiphilus sp. nov. is proposed; the type strain is YIT 11850(T) (=DSM 21274(T)=JCM 15077(T)). Cells of the other isolate, strain YIT 11860(T), were non-motile, rod-shaped, positive for aesculin hydrolysis, negative for indole production, produced succinic and acetic acids as end products of glucose metabolism and possessed a DNA G+C content of 45.5 mol%. On the basis of 16S rRNA gene sequence similarity values, this strain was shown to belong to the family 'Porphyromonadaceae' related to Barnesiella viscericola (96.0 %); similarity values with species within the family 'Porphyromonadaceae' with validly published names were less than 86 %. Biochemical data supported the affiliation of strain YIT 11860(T) to the genus Barnesiella. Strain YIT 11860(T) therefore represents a novel species for which the name Barnesiella intestinihominis sp. nov. is proposed; the type strain is YIT 11860(T) (=DSM 21032(T)=JCM 15079(T)).
从人类粪便中分离出两株革兰氏阴性、厌氧、无芽孢形成的细菌（YIT 11850(T)和YIT 11860(T)）。菌株YIT 11850(T)的细胞为球杆菌，不分解糖类且基本无反应，代谢终产物仅有微量乳酸和丙酸；然而，菌株YIT 11850(T)能够将琥珀酸脱羧生成丙酸。菌株YIT 11850(T)的DNA G+C含量为51.9 mol%。经过16S rRNA基因序列分析，发现该菌株与丙酸迪氏菌关系最为密切，两个分类单元之间的序列相似性为95.1%。生化数据支持菌株YIT 11850(T)归属于迪氏菌属。因此，菌株YIT 11850(T)代表一个新物种，提议将其命名为琥珀酸迪氏菌（Dialister succinatiphilus sp. nov.）；模式菌株为YIT 11850(T)（=DSM 21274(T)=JCM 15077(T)）。另一分离株YIT 11860(T)的细胞无运动性，呈杆状，七叶苷水解呈阳性，吲哚产生呈阴性，葡萄糖代谢的终产物为琥珀酸和乙酸，DNA G+C含量为45.5 mol%。基于16S rRNA基因序列相似性值，该菌株被证明属于与内脏巴内西菌相关的“卟啉单胞菌科”（96.0%）；与有效发表名称的“卟啉单胞菌科”内物种的相似性值小于86%。生化数据支持菌株YIT 11860(T)归属于巴内西菌属。因此，菌株YIT 11860(T)代表一个新物种，提议将其命名为人类肠道巴内西菌（Barnesiella intestinihominis sp. nov.）；模式菌株为YIT 11860(T)（=DSM 21032(T)=JCM 15079(T)）。
