Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 186-8650 Tokyo, Japan.
Int J Syst Evol Microbiol. 2010 Nov;60(Pt 11):2639-2646. doi: 10.1099/ijs.0.017533-0. Epub 2009 Dec 18.
Three Gram-positive, strictly anaerobic, non-spore-forming, rod-shaped organisms (strains YIT 12062(T), YIT 12063(T) and YIT 12064) were isolated from human faeces. Strain YIT 12062(T) was asaccharolytic and possessed a DNA G+C content of 58.3 mol%. Cells of strain YIT 12062(T) were negative for catalase, oxidase, urease, hydrolysis of aesculin and gelatin, nitrate reduction and indole production. Based on 16S rRNA gene sequence analysis, strain YIT 12062(T) was assigned to the genus Slackia (91.7-96.0 % sequence similarities to type strains of Slackia species). Biochemical data showed that the isolate was phenotypically distinct from all recognized species of the genus Slackia. Strain YIT 12062(T) therefore represents a novel species in the genus Slackia, for which the name Slackia piriformis sp. nov. is proposed. The type strain is YIT 12062(T) (=DSM 22477(T)=JCM 16070(T)). Following 16S rRNA gene sequence analysis, strains YIT 12063(T) and YIT 12064, which were isolated from different subjects, were shown to be most closely related to species of the genus Collinsella (93.8-95.1 % similarities to type strains). Although their phenotypic characteristics were very similar and they shared >99 % 16S rRNA gene sequence similarity and >97±1.8 % DNA-DNA relatedness, the two isolates could be discriminated by RAPD fingerprints. The DNA G+C contents of strains YIT 12063(T) and YIT 12064 were 60.8 and 61.0 mol%, respectively. They were saccharolytic in API test systems, positive for aesculin hydrolysis and negative for catalase, oxidase, urease, indole production, nitrate reduction and gelatin hydrolysis. The major end products of glucose fermentation of these strains were lactate, acetate and formate. Biochemical data supported the affiliation of strains YIT 12063(T) and YIT 12064 to the genus Collinsella and showed that they were phenotypically distinct from all recognized species of the genus Collinsella. Strains YIT 12063(T) and YIT 12064 therefore represent a novel species of the genus Collinsella, for which the name Collinsella tanakaei sp. nov. is proposed. The type strain is YIT 12063(T) (=DSM 22478(T)=JCM 16071(T)).
从人类粪便中分离到三株革兰氏阳性、严格厌氧、不产芽孢的杆状菌（菌株 YIT 12062(T)、YIT 12063(T) 和 YIT 12064）。菌株 YIT 12062(T) 为非发酵糖，其 DNA G+C 含量为 58.3 mol%。菌株 YIT 12062(T) 的细胞为过氧化氢酶阴性、氧化酶阴性、脲酶阴性、马栗树皮苷水解阴性、明胶水解阴性、硝酸盐还原阴性和吲哚产生阴性。基于 16S rRNA 基因序列分析，菌株 YIT 12062(T) 被归为 Slackia 属（与 Slackia 属的模式种的序列相似度为 91.7-96.0%）。生化数据表明，该分离株与 Slackia 属的所有已知种在表型上均有明显不同。因此，菌株 YIT 12062(T) 代表了 Slackia 属中的一个新种，建议将其命名为 Slackia piriformis sp. nov.。模式菌株为 YIT 12062(T)（=DSM 22477(T)=JCM 16070(T)）。根据 16S rRNA 基因序列分析，从不同个体中分离到的菌株 YIT 12063(T) 和 YIT 12064 与 Collinsella 属的种最为密切相关（与模式种的相似度为 93.8-95.1%）。尽管它们的表型特征非常相似，且 16S rRNA 基因序列相似度>99%，DNA-DNA 同源性>97±1.8%，但通过 RAPD 指纹图谱可以区分这两个分离株。菌株 YIT 12063(T) 和 YIT 12064 的 DNA G+C 含量分别为 60.8 和 61.0 mol%。它们在 API 测试系统中为发酵糖，马栗树皮苷水解阳性，过氧化氢酶、氧化酶、脲酶、吲哚产生、硝酸盐还原和明胶水解阴性。这些菌株葡萄糖发酵的主要终产物为乳酸、乙酸和甲酸盐。生化数据支持菌株 YIT 12063(T) 和 YIT 12064 与 Collinsella 属的相关性，并表明它们在表型上与 Collinsella 属的所有已知种均有明显不同。因此，菌株 YIT 12063(T) 和 YIT 12064 代表了 Collinsella 属的一个新种，建议将其命名为 Collinsella tanakaei sp. nov.。模式菌株为 YIT 12063(T)（=DSM 22478(T)=JCM 16071(T)）。
