Ogasawara Shinzi, Maeda Mizuo
RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.
Nucleic Acids Symp Ser (Oxf). 2009(53):195-6. doi: 10.1093/nass/nrp098.
We have successfully developed a new method for photoregulation of G-quadruplex formation using cis-trans photoisomerization of the photochromic nucleobase (8FV)G. Our photo-controllable quadruplexes can be switched between a very stable quadruplex state and a non-structured state in a straightforward and reversible fashion. We also demonstrated reversibly control binding of a G-quadruplex aptamer to thrombin.
我们已成功开发出一种利用光致变色核碱基(8FV)G的顺反光异构化对G-四链体形成进行光调控的新方法。我们的光控四链体能够以直接且可逆的方式在非常稳定的四链体状态和非结构化状态之间切换。我们还展示了对G-四链体适配体与凝血酶结合的可逆控制。
