Sands T W, Petras M L, Van Wijngaarden J
Department of Biological Sciences, University of Windsor, Ontario, Canada.
Int J Biomed Comput. 1990 Jul;26(1-2):39-52. doi: 10.1016/0020-7101(90)90018-p.
Type II restriction endonucleases cleave double stranded DNA molecules at sites characterized by one or more sets of nucleotide pairs sequences. These digestions are essential in such procedures as DNA cloning, DNA sequencing and restriction fragment length polymorphism (RFLP) analyses. A large number of enzymes with different sequence specificities are available. To date, most choices of restriction endonucleases have been made by trial and error. A computer program, REDI, has been developed that predicts the ability of a particular restriction enzyme to detect mutations. Characteristics of both the restriction endonuclease used and the DNA being cut are incorporated as variables in the program. The program was tested using mouse mitochondrial DNA (mtDNA) and bacteriophage lambda DNA because these have been sequenced and are well characterized. REDI was strongly correlated (rs = +0.862, n = 11, P less than 0.001) with mouse mtDNA RFLP detected by Ferris et al. [1] (Genetics, 105 (1983) 681-721). Even though predictions may be altered by a non-random association of nucleotides, which varies among DNA molecules, the predictions increase the probability of selecting the most efficient enzymes for use in the analysis of a particular DNA molecule.
II型限制性核酸内切酶在以一组或多组核苷酸对序列为特征的位点切割双链DNA分子。这些消化作用在DNA克隆、DNA测序和限制性片段长度多态性（RFLP）分析等程序中至关重要。有大量具有不同序列特异性的酶可供使用。迄今为止，大多数限制性核酸内切酶的选择都是通过反复试验做出的。已经开发了一个计算机程序REDI，它可以预测特定限制性酶检测突变的能力。所用限制性核酸内切酶和被切割DNA的特征都作为变量纳入该程序。该程序使用小鼠线粒体DNA（mtDNA）和噬菌体λDNA进行了测试，因为这些已经被测序且特征明确。REDI与Ferris等人[1]（《遗传学》，105（1983）681 - 721）检测到的小鼠mtDNA RFLP高度相关（rs = +0.862，n = 11，P小于0.001）。尽管预测可能会因DNA分子之间核苷酸的非随机关联而改变，但这些预测增加了选择最有效酶用于分析特定DNA分子的可能性。
