Ito J, Roberts R J
Gene. 1979 Jan;5(1):1-7. doi: 10.1016/0378-1119(79)90088-x.
Susceptibility of Bacillus subtilis phage phi 29 DNA to 34 different restriction endoculceases was determined. Three enzymes, BglI, XbaI and BstEII, were found to cleave phi 29 DNA only once at specific sites. The sites of these single cleavages have been mapped. Thirteen enzymes did not cut phi 29 DNA. phi 29 HindIII DNA fragments inserted into pBR313 plasmid and propagated in Escherichia coli, were resistant to these restriction endonucleases. This result suggests that the insusceptibility is due to the absence of the nucleotide sequences on phi 29 recognized by the enzymes, and not to the presence of modified nucleotides.
测定了枯草芽孢杆菌噬菌体φ29 DNA对34种不同限制性内切酶的敏感性。发现三种酶BglI、XbaI和BstEII仅在特定位点切割φ29 DNA一次。这些单一切割的位点已被定位。13种酶不切割φ29 DNA。插入pBR313质粒并在大肠杆菌中繁殖的φ29 HindIII DNA片段对这些限制性内切酶具有抗性。这一结果表明，不敏感性是由于φ29上不存在被这些酶识别的核苷酸序列，而不是由于存在修饰的核苷酸。
