Salih M A, Danielsson D, Bäckman A, Caugant D A, Achtman M, Olcén P
Department of Paediatrics and Child Health, Faculty of Medicine, University of Khartoum, Sudan.
J Clin Microbiol. 1990 Aug;28(8):1711-9. doi: 10.1128/jcm.28.8.1711-1719.1990.
A random selection of 25 strains isolated during an epidemic caused by serogroup A Neisseria meningitidis in Sudan (1988), 3 preepidemic meningococcal strains (1985), and 26 serogroup A strains isolated from sporadic cases of meningitis in Sweden (1973 to 1987) were assessed for multilocus enzyme genotypes (ETs), DNA restriction enzyme patterns, outer membrane proteins, lipopolysaccharides, pilus formation, and antibiograms. All of the 25 Sudanese epidemic isolates and 22 of the Swedish strains were of the same or closely related ETs (ETs 3, 4, and 5), corresponding to clone III-1, which has been responsible for two pandemic waves in the last three decades. The earlier pandemic involved Scandinavia, and the last one caused an outbreak during the pilgrimage to Mecca, Saudi Arabia (August 1987), spreading to Sudan, Chad, and Ethiopia. The three Sudanese preepidemic isolates (1985) were clone IV-1 (sulfonamide susceptible), which has been resident in the African meningitis belt for the last 25 years. The uniformity of clone III-1 strains (all sulfonamide resistant) from Sudan and Sweden was confirmed by DNA restriction enzyme patterns. ETs 3, 4, and 5 from Sudan and Sweden had 86 to 100% similarity to a Swedish clone III-1 reference strain, whereas ETs 1, 2, 6, and 7 showed 50 to 80% similarity. Class 1 protein for clone III-1 showed serosubtype antigens P1.9 and P1.x, whereas ET6 strains (clone IV-1) had serosubtype P1.7. Lipopolysaccharides were variable in the Sudanese and Swedish strains. Pili were expressed in all clone III-1 isolates from Sudan and Sweden but in none of the clone IV-1 isolates (Sudan, 1985).
随机选取了在苏丹（1988年）由A群脑膜炎奈瑟菌引起的一次流行期间分离出的25株菌株、3株流行前的脑膜炎球菌菌株（1985年）以及从瑞典（1973年至1987年）散发性脑膜炎病例中分离出的26株A群菌株，对其多位点酶基因型（ETs）、DNA限制性酶切图谱、外膜蛋白、脂多糖、菌毛形成及抗菌谱进行了评估。25株苏丹流行株中的所有菌株以及22株瑞典菌株属于相同或密切相关的ETs（ETs 3、4和5），对应于III-1克隆，该克隆在过去三十年中引发了两次大流行浪潮。较早的一次大流行涉及斯堪的纳维亚半岛，最近一次在前往沙特阿拉伯麦加朝圣期间（1987年8月）引发了疫情，并蔓延至苏丹、乍得和埃塞俄比亚。三株苏丹流行前分离株（1985年）为IV-1克隆（对磺胺类药物敏感），在过去25年一直存在于非洲脑膜炎带。通过DNA限制性酶切图谱证实了来自苏丹和瑞典的III-1克隆菌株（均对磺胺类药物耐药）的一致性。来自苏丹和瑞典的ETs 3、4和5与一株瑞典III-1克隆参考菌株的相似性为86%至100%，而ETs 1、2、6和7的相似性为50%至80%。III-1克隆的1类蛋白显示血清亚型抗原P1.9和P1.x，而ET6菌株（IV-1克隆）具有血清亚型P1.7。苏丹和瑞典菌株中的脂多糖存在差异。苏丹和瑞典的所有III-1克隆分离株均表达菌毛，但IV-1克隆分离株（苏丹，1985年）均未表达菌毛。
