一种快速简便的放射性方法，用于从单一样本的生物流体或组织中测定双硫仑及其代谢物。

A rapid and simple radioactive method for the determination of disulfiram and its metabolites from a single sample of biological fluid or tissue.

作者信息

Faiman M D, Dodd D E, Nolan R J, Artman L, Hanzlik R E

出版信息

Res Commun Chem Pathol Pharmacol. 1977 Jul;17(3):481-96.

PMID:197575

Abstract

An analytical method is described for the determination of radioactive disulfiram, diethyldithiocarbamate, diethyldithiocarbamate-methyl ester, diethyldithiocarbamate-glucuronide, inorganic sulfate, and a protein bound S35 fraction from a single sample of either plasma, urine or tissue. The procedure is based upon quantitative stepwise extraction or precipitation of the individual compounds, and is both specific and precise. The applicability of the methods developed for the determination of S35 disulfiram and its S35 metabolites in plasma and urine from a dog given S35 disulfiram i.v., and in mouse brain from mice given S35 disulfiram i.p. are illustrated.

摘要

本文描述了一种分析方法，用于测定来自血浆、尿液或组织的单个样品中的放射性双硫仑、二乙基二硫代氨基甲酸盐、二乙基二硫代氨基甲酸盐甲酯、二乙基二硫代氨基甲酸盐葡糖醛酸苷、无机硫酸盐以及与蛋白质结合的S35组分。该方法基于对各个化合物进行定量逐步萃取或沉淀，具有特异性和精确性。文中举例说明了所开发方法在测定静脉注射S35双硫仑的犬的血浆和尿液以及腹腔注射S35双硫仑的小鼠的小鼠脑中的S35双硫仑及其S35代谢物方面的适用性。