Department of Radiation Oncology.
Department of Oncology.
Ann Oncol. 2010 Mar;21(3):589-596. doi: 10.1093/annonc/mdp333. Epub 2009 Sep 16.
The purpose of this study was to investigate prognostic significance of fascin1 in nasopharyngeal carcinoma (NPC) and evaluate the association of fascin1 with tumor invasion.
Immunohistochemical staining for fascin1 was carried out on paraffin-embedded tissue sections from 161 patients with NPC. Data were subjected to statistical analysis with respect to clinicopathological variables and survival. Small interfering RNA (siRNA) approach was used to knockdown fascin1 expression in NPC cells to determine whether fascin1 contributes to tumor cell invasion.
Immunohistochemical analysis showed that fascin1 was highly expressed in 95 (59.0%) of 161 paraffin-embedded NPC tissues. Fascin1 expression was significantly correlated with clinical stage (P < 0.001) and N classification (P < 0.001). Statistical analysis showed that fascin1 expression was inversely correlated with both overall and disease-free survival of NPC patients. Multivariate analysis showed that fascin1 expression was an independent prognostic indicator for patient's survival. Moreover, disruption of endogenous fascin1 expression in NPC cells using siRNA technique suppressed NPC cell invasiveness and decreased cell filopodia and lamellopodia.
The present study indicates that fascin1 expression is inversely correlated with NPC patient survival and directly correlated with the malignant status of NPC.
本研究旨在探讨 fascin1 在鼻咽癌（NPC）中的预后意义，并评估 fascin1 与肿瘤侵袭的相关性。
对 161 例 NPC 患者的石蜡包埋组织切片进行 fascin1 的免疫组织化学染色。对临床病理变量和生存数据进行统计分析。采用小干扰 RNA（siRNA）方法敲低 NPC 细胞中的 fascin1 表达，以确定 fascin1 是否有助于肿瘤细胞侵袭。
免疫组织化学分析显示，在 161 例 NPC 石蜡包埋组织中有 95 例（59.0%）高表达 fascin1。fascin1 的表达与临床分期（P<0.001）和 N 分类（P<0.001）显著相关。统计学分析显示，fascin1 的表达与 NPC 患者的总生存和无病生存呈负相关。多因素分析表明，fascin1 的表达是 NPC 患者生存的独立预后指标。此外，采用 siRNA 技术破坏 NPC 细胞内源性 fascin1 表达，抑制 NPC 细胞侵袭，并减少细胞丝状伪足和片状伪足。
本研究表明，fascin1 的表达与 NPC 患者的生存呈负相关，与 NPC 的恶性状态直接相关。
