克麦罗沃病毒双链RNA片段5'-末端修饰的证据及其经碱性磷酸酶处理后的去除情况。
Evidence of 5'-terminal modification in the kemerovo virus double-stranded RNA segments and its removal by treatment with alkaline phosphatase.
作者信息
Bacík I
机构信息
Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
出版信息
Acta Virol. 1990 Apr;34(2):193-7.
PMID:1975982
Abstract
Only one strand of each double-stranded (ds) RNA segment of the Kemerovo virus genome was 5'end-labelled using gamma-32P-ATP and T 4 polynucleotide kinase after preceding dephosphorylation of 5'ends by calf intestinal alkaline phosphatase. This suggests a 5'-terminal modification of the one of complementary strands in the ds RNA segments.
摘要
在用小牛肠碱性磷酸酶对科米罗夫病毒基因组的每个双链（ds）RNA片段的5'端进行去磷酸化处理后，使用γ-32P-ATP和T4多核苷酸激酶对其中一条链的5'端进行了放射性标记。这表明ds RNA片段中互补链之一存在5'端修饰。
Zotero 插件