Brown S E, Morrison H G, Knudson D L
Department of Entomology, College of Agricultural Sciences, Colorado State University, Fort Collins 80523.
Acta Virol. 1989 May;33(3):206-20.
The majority of the thirty-two Great Island serocomplex isolates examined exhibit distinct dsRNA polyacrylamide gel profiles. Yet, these viruses are closely related by blot hybridization with only two genes showing significant sequence divergence. Gene reassortment was demonstrated between selected pairs of the Great Island serocomplex viruses with two different geographic regions represented. The majority of the reassortant progeny from the cross of selected pairs resulted in progeny with multiple gene-replacements. The ability of these selected isolates to reassort confirms the close taxonomic relationship of the isolates in spite of their geographic distribution.
在所检测的32株大岛血清复合体分离株中，大多数呈现出独特的双链RNA聚丙烯酰胺凝胶图谱。然而，通过印迹杂交表明这些病毒密切相关，只有两个基因存在显著的序列差异。在具有两个不同地理区域代表性的大岛血清复合体病毒的选定配对之间证实了基因重配。选定配对杂交产生的大多数重配后代产生了具有多个基因替换的后代。这些选定分离株进行重配的能力证实了尽管分离株存在地理分布差异，但它们在分类学上关系密切。
