人类鸟氨酸脱羧酶基因序列的两个染色体定位及在结直肠肿瘤中的高表达
Two chromosomal locations for human ornithine decarboxylase gene sequences and elevated expression in colorectal neoplasia.
作者信息
Radford D M, Nakai H, Eddy R L, Haley L L, Byers M G, Henry W M, Lawrence D D, Porter C W, Shows T B
机构信息
Department of Surgical Oncology, Roswell Park Memorial Institute, New York State Department of Health, Buffalo 14263.
出版信息
Cancer Res. 1990 Oct 1;50(19):6146-53.
The polyamines are known to be essential for cellular proliferation. Ornithine decarboxylase (ODC) is a rate-limiting enzyme in the synthesis of these amines, and activity is elevated in colorectal tumors and polyps. Two ODC genes (designated ODC1 and ODC2) were localized by somatic cell hybridization and in situ techniques to 2p25 and 7q31-qter, respectively. Investigation of the expression of ODC in colorectal neoplasia reveals a consistent increase in mRNA expression compared with normal adjacent mucosa and control mucosa, ranging from 1.3- to 12.2-fold. No amplification of the loci was seen. Comparison of ODC mRNA expression with ODC activity from the same samples revealed no direct correlation, suggesting that regulation of ODC in this system occurs at the posttranscriptional level.
已知多胺对细胞增殖至关重要。鸟氨酸脱羧酶（ODC）是这些胺类合成中的限速酶，其活性在结直肠癌和息肉中升高。通过体细胞杂交和原位技术分别将两个ODC基因（命名为ODC1和ODC2）定位到2p25和7q31 - qter。对结直肠肿瘤中ODC表达的研究表明，与相邻正常黏膜和对照黏膜相比，mRNA表达持续增加，范围为1.3至12.2倍。未观察到基因座的扩增。对同一样本的ODC mRNA表达与ODC活性进行比较，未发现直接相关性，这表明该系统中ODC的调节发生在转录后水平。
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