Conway P L, Welin A, Cohen P S
Department of General and Marine Microbiology, University of Goteborg, Sweden.
Infect Immun. 1990 Oct;58(10):3178-82. doi: 10.1128/iai.58.10.3178-3182.1990.
Ileal mucus and epithelial cells were isolated from newborn piglets that had never been fed and 35-day-old unweaned piglets. Both newborn and 35-day-old piglet mucus preparations supported growth of Escherichia coli Bd 1107/75 08, a K88-fimbriated porcine enterotoxigenic strain, equally well (i.e., generation times of 28 min were observed in both cases). Adhesion of E. coli Bd 1107/75 08 to 35-day-old piglet ileal epithelial cells was, at most, 2 times that of the same strain to newborn piglet ileal epithelial cells; however, adhesion of E. coli Bd 1107/75 08 to 35-day-old piglet ileal mucus was 16 times that of the same strain to newborn piglet ileal mucus. The receptor in 35-day-old piglet ileal mucus was K88 specific, since it could be removed by purified K88ab fimbriae. Furthermore, adhesion of E. coli Bd 1107/75 08 to 35-day-old piglet ileal mucus was blocked by PAB10, a K88ab-, K88ac-, K88ad-specific monoclonal antibody. Although E. coli Bd 1107/75 08 traversed both newborn and 35-day-old piglet ileal mucus about equally well in vitro and bound well to underlying ileal epithelial cells after passing through newborn ileal mucus, it did not bind to ileal epithelial cells after passing through 35-day-old piglet ileal mucus. The data are discussed with respect to the role that K88-specific receptors present in newborn and ileal mucus might play in the pathogenesis of porcine enterotoxigenic E. coli strains which bear K88 fimbriae.
从从未进食过的新生仔猪和35日龄未断奶仔猪中分离出回肠黏液和上皮细胞。新生仔猪和35日龄仔猪的黏液制剂对大肠杆菌Bd 1107/75 08（一种K88菌毛化的猪产肠毒素菌株）的生长支持效果相同（即两种情况下观察到的代时均为28分钟）。大肠杆菌Bd 1107/75 08对35日龄仔猪回肠上皮细胞的黏附力，最多是该菌株对新生仔猪回肠上皮细胞黏附力的2倍；然而，大肠杆菌Bd 1107/75 08对35日龄仔猪回肠黏液的黏附力是该菌株对新生仔猪回肠黏液黏附力的16倍。35日龄仔猪回肠黏液中的受体具有K88特异性，因为它可被纯化的K88ab菌毛去除。此外，大肠杆菌Bd 1107/75 08对35日龄仔猪回肠黏液的黏附被PAB10（一种K88ab、K88ac、K88ad特异性单克隆抗体）阻断。尽管大肠杆菌Bd 1107/75 08在体外穿过新生仔猪和35日龄仔猪回肠黏液的能力大致相同，且在穿过新生回肠黏液后能很好地结合到下层回肠上皮细胞，但在穿过35日龄仔猪回肠黏液后却不与回肠上皮细胞结合。文中讨论了新生和回肠黏液中存在的K88特异性受体可能在携带K88菌毛的猪产肠毒素大肠杆菌菌株发病机制中所起的作用。
